Resinoid of Cistus ladaniferus (Cistaceae) obtained from labdanum gum by organic solvents extraction

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06-10 November 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The substance is considered to be adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Version / remarks:

2012

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

inspected on 15-16 November 2017 / signed on 15 May 2018

Type of method:

pycnometer method

Remarks:

with replacement liquid

Key result

Type:

density

Density:

1.045 g/cm³

Temp.:

20 °C

Remarks on result:

other:

Remarks:

mean of two independent determinations

Key result

Type:

relative density

Density:

1.045 other: unitless

Temp.:

20 °C

Remarks on result:

other:

Remarks:

mean of two independent determinations

Table 4.4/1: Measured Values

 

First Determination				Error rel.	Error abs.
Temperature	20.2	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn. + H2O	Pycn. + repl.liquid	Pycn. + test item	Pycn. + test item + repl. liquid
Mass in g	36.7398	61.6067	61.6033	44.2492	61.9455
Temperature in °C	20.2	20.2	19.8	20.2	20.2
Volume of Pycnometer	24.9117	cm^3
Density of Replacement liquid	0.9981	g/cm^3		Error rel.	Error abs.
Density of Test Item	1.0457	g/cm^3		0.0296	0.0003
Second Determination				Error rel.	Error abs.
Temperature	20.0	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn. + H2O	Pycn. + repl.liquid	Pycn. + test item	Pycn. + test item + repl. liquid
Mass in g	36.7411	61.6033	61.5994	45.9604	62.0096
Temperature in °C	20.4	20.0	19.8	20.0	20.0
Volume of Pycnometer	24.9070	cm^3
Density of Replacement liquid	0.9980	g/cm^3		Error rel.	Error abs.
Density of Test Item	1.0445	g/cm^3		0.0250%	0.0003

Results of the single determinations with the respective error as well as the mean value and its standard deviation are presented in the following tables:

Table 4.4/2: Results of Single Determinations

 

Determination	Density in g/cm^3	Error of Determ. in g/cm^3
1	1.0457	0.0003
2	1.0445	0.0003

 

Table 4.4/3 Results – Mean Values

 

Parameter	Value	Unit	Standard Deviation	Relative Standard Deviation
Volume of Pycnometer	24.91	cm^3	0.0033	0.01%
Density of Test Item	1.0451	g/ cm^3	0.0008	0.08%
Density of Test Item	1045.1	kg/m^3	0.8	0.08%
Relative Density of Test Item (D20/4)	1.0451		0.0008	0.08%

The density of the test item was calculated as 1.0451 ± 0.0003 g/cm³ at 20.0 ± 0.4 °C, which is the mean of two independent determinations ± the standard deviation. Standard deviation shows good correlation of the two determinations. Therefore, the study is considered valid.

Conclusions:

The density of the test substance was measured as 1.0451 ± 0.0003 g/cm3 at 20.0 ± 0.4 °C and the relative density is determined as D20/4 = 1.0451
Denser than water

Executive summary:

The density of the test substance was measured under GLP according OECD 109 / EU A3 guideline, using the pycnometer method with a replacement liquid.

Two determinations were performed and the mean value was retained.

This result was then converted to relative density.

Mean density: 1.0451 g/cm³at 20.0 ± 0.4 °C

Mean relative density (D20/4) : 1.0451

Description of key information

The density of the test item was measured as 1.0451 ± 0.0003 g/cm3 at 20.0 ± 0.4 °C and the relative density is determined as D20/4 = 1.0451

Key value for chemical safety assessment

Relative density at 20C:

1.045

Additional information

A fully reliable experimental study, conducted under GLP according to OECD 109 /EU A3 guideline, using pycnometer method, is available. It is considered as a key study, and the result is retained as key data.