Registration Dossier
Registration Dossier
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EC number: 226-237-1 | CAS number: 5332-24-1
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- data from handbook or collection of data
Data source
Reference
- Reference Type:
- review article or handbook
- Title:
- determination of Skin sensitization for CAS no: 100-00-5
- Author:
- HSDB
- Year:
- 2 017
- Bibliographic source:
- U.S. National Library of Medicine National Institutes of Health, Health & Human Services; 2017.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- The Mouse ear swelling test was performed on five mice to assess the skin sensitization potential of chemical 1-Chloro-4-nitrobenzene (CAS no: 100-00-5).
- GLP compliance:
- not specified
- Type of study:
- mouse ear swelling test
- Justification for non-LLNA method:
- not specified
Test material
- Reference substance name:
- 1-chloro-4-nitrobenzene
- EC Number:
- 202-809-6
- EC Name:
- 1-chloro-4-nitrobenzene
- Cas Number:
- 100-00-5
- Molecular formula:
- C6H4ClNO2
- IUPAC Name:
- 1-Chloro-4-nitrobenzene
- Details on test material:
- - IUPAC Name: 1-Chloro-4-nitrobenzene
- Common Name: 4-Chloronitrobenzene
- Mol. formula: C6H4ClNO2
- Molecular Weight: 157.556 g/mole
- Smiles: c1([N+](=O)[O-])ccc(Cl)cc1
- InChI: 1S/C6H4ClNO2/c7-5-1-3-6(4-2-5)8(9)10/h1-4H
Constituent 1
- Specific details on test material used for the study:
- - IUPAC Name: 1-Chloro-4-nitrobenzene
- Common Name: 4-Chloronitrobenzene
- Mol. formula: C6H4ClNO2
- Molecular Weight: 157.556 g/mole
- Smiles: c1([N+](=O)[O-])ccc(Cl)cc1
- InChI: 1S/C6H4ClNO2/c7-5-1-3-6(4-2-5)8(9)10/h1-4H
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals and environmental conditions:
- No data available
Study design: in vivo (non-LLNA)
Induction
- Route:
- other: epicutaneous
- Vehicle:
- other: acetone
- Concentration / amount:
- 0.2 mL of a 0.05 M solution
- Day(s)/duration:
- No data available
- Adequacy of induction:
- not specified
Challenge
- No.:
- #1
- Route:
- other: epicutaneous
- Vehicle:
- other: Acetone
- Concentration / amount:
- 20 uL of 0.016 M solution
- Day(s)/duration:
- 72 hours
- Adequacy of challenge:
- highest non-irritant concentration
- No. of animals per dose:
- 5
- Details on study design:
- MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: 1
- Exposure period: no data
- Test groups: 5 mice
- Control group: no data
- Site: shaved back of each mice
- Frequency of applications: no data
- Duration: no data
- Concentrations: 0.2 mL of a 0.05 M solution
B. CHALLENGE EXPOSURE
- No. of exposures: 1
- Day(s) of challenge: on day 5
- Exposure period: 24 hours
- Test groups: 5 mice
- Control group: o data
- Site: the surface of one ear of each
mouse
- Concentrations: 20 uL of 0.016 M solution
- Evaluation (hr after challenge): 24 , 48 and 72 hours - Challenge controls:
- Controls were used but detail information was not mentioned.
Results and discussion
In vivo (non-LLNA)
Results
- Reading:
- 1st reading
- Hours after challenge:
- 72
- Group:
- test chemical
- Dose level:
- 20 uL of 0.016 M solution
- No. with + reactions:
- 0
- Total no. in group:
- 5
- Clinical observations:
- No skin sensitization was observed.
- Remarks on result:
- no indication of skin sensitisation
In vivo (LLNA)
- Cellular proliferation data / Observations:
- No skin sensitization was observed.
Applicant's summary and conclusion
- Interpretation of results:
- other: Not sensitizing
- Conclusions:
- The test material 1-Chloro-4-nitrobenzene (CAS no: 100-00-5) was considered to be not sensitizing to the skin of mice.
- Executive summary:
The Mouse ear swelling test was performed on five mice to assess the skin sensitization potential of chemical 1-Chloro-4-nitrobenzene (CAS no: 100-00-5).
The backs of 5 mice were shaved with electric clippers before induction was performed by applying 0.2 mL of a 0.05 M solution of the compound in acetone on day 1. Challenge application was performed with 20 uL of non-irritant 0.016 M solution to the surface of one ear of each mouse on day 5.
Reactions were read after a further 24, 48 and 72 hours by comparison of the thickness of the ears between test mice and controls.
Since no known effects were observed, the chemical1-Chloro-4-nitrobenzene (CAS no: 100-00-5) was considered to be not sensitizing to the skin of mice.
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