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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
data from peer- reviewed journals

Data source

Reference
Reference Type:
review article or handbook
Title:
A Review of the Toxicity of Twelve Fluorescent Dyes Used for Water Tracing
Author:
P.L Smart
Year:
1984
Bibliographic source:
The NSS Bulletin, Volume 46 Number 2: 21-33 - October 1984, A publication of the National Speleological Society

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
To assess the ocular irritation potential of Pyranine
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Trisodium 8-hydroxypyrene-1,3,6-trisulphonate
EC Number:
228-783-6
EC Name:
Trisodium 8-hydroxypyrene-1,3,6-trisulphonate
Cas Number:
6358-69-6
Molecular formula:
C16H10O10S3.3Na
IUPAC Name:
trisodium 8-hydroxypyrene-1,3,6-trisulfonate
Test material form:
solid: particulate/powder
Details on test material:
Name of test material (as cited in study report): Solvent Green 7 also known as Pyranine
Molecular formula : C16H10O10S3.3Na
Molecular weight : 524.385088 g/mol
Smiles notation : c12c3c4c(cc(c3ccc2c(S([O])(=O)=O)cc(c1cc4)S([O])(=O)=O)O)S([O])(=O)=O.[Na+].[Na+].[Na+]
InChl: 1S/C16H10O10S3.3Na/c1711512(27(18,19)20)8341014(29(24,25)26)613(28(21,22)23)9217(11)15(8)16(9)10;;;/h16,17H,(H,18,19,20)(H,21,22,23)(H,24,25,26);;;/q;3*+1/p3
Substance type: Organic
Physical State: Solid
Specific details on test material used for the study:
Name of test material (as cited in study report): Solvent Green 7 also known as Pyranine
Molecular formula : C16H10O10S3.3Na
Molecular weight : 524.385088 g/mol
Smiles notation : c12c3c4c(cc(c3ccc2c(S([O])(=O)=O)cc(c1cc4)S([O])(=O)=O)O)S([O])(=O)=O.[Na+].[Na+].[Na+]
InChl: 1S/C16H10O10S3.3Na/c1711512(27(18,19)20)8341014(29(24,25)26)613(28(21,22)23)9217(11)15(8)16(9)10;;;/h16,17H,(H,18,19,20)(H,21,22,23)(H,24,25,26);;;/q;3*+1/p3
Substance type: Organic
Physical State: Solid

Test animals / tissue source

Species:
other: mammals
Strain:
not specified
Details on test animals or tissues and environmental conditions:
no data

Test system

Vehicle:
not specified
Controls:
not specified
Amount / concentration applied:
no data
Duration of treatment / exposure:
no data
Observation period (in vivo):
no data
Number of animals or in vitro replicates:
no data
Details on study design:
no data

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: no data available
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
No signs of irritation observed

Applicant's summary and conclusion

Interpretation of results:
other: not irritating
Conclusions:
Pyranine was observed to be non – irritating to eyes. Since there was no evidence of substantial toxicity, it was considered that Pyranine can be used safely for the angiography in the human eye.
Executive summary:

In an ocular irritation study conducted on mammals (species - not mentioned), Pyranine was observed to be non – irritating to eyes.Since there was no evidence of substantial toxicity, it was considered that Pyranine can be used safely for the angiography in the human eye.