4,4',4''-triaminotrityl alcohol

Endpoint summary

Description of key information

Skin irritation:

The dermal irritation potential of 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) was estimated using OECD QSAR toolbox version 3.4 with logPow as the primary descriptor. The substance 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) was estimated to be not irritating to the skin of New Zealand White rabbits. Based on the estimated result 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) can be considered to be not irritating to skin and can be classified under the category ˋ Not Classified’ as per CLP regulation.

 

Eye irritation:

The ocular irritation potential of 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) was estimated using OECD QSAR toolbox version 3.4 with logPow as the primary descriptor. The substance 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) was estimated to be not irritating into the eyes of New Zealand White rabbits. Based on the estimated result 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) can be considered to be not irritating to eye and can be classified under the category ˋ Not Classified’ as per CLP regulation.

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records

Reference

Endpoint:

skin irritation: in vivo

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification

Justification for type of information:

Data is from OECD QSAR toolbox version 3.4 and QMRF report has been attached

Principles of method if other than guideline:

Prediction is done using QSAR Toolbox version 3.4

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): 4,4',4''-Triaminotrityl alcohol
- Molecular formula: C19H19N3O
- Molecular weight: 305.379 g/mol
- Smiles notation: C(c1ccc(N)cc1)(c1ccc(N)cc1)(c1ccc(N)cc1)O
-InChl: 1S/C19H19N3O/c20-16-7-1-13(2-8-16)19(23,14-3-9-17(21)10-4-14)15-5-11-18(22)12-6-15/h1-12,23H,20-22H2
- Substance type: Organic
- Physical state: Solid

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

No data available

Type of coverage:

semiocclusive

Preparation of test site:

shaved

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

No data available

Duration of treatment / exposure:

4 hours

Observation period:

72 hours

Number of animals:

3

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

72 h

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

no skin reaction was observed.

Estimation method: Takes mode value from the 6 nearest neighbours
Domain  logical expression:Result: In Domain

(((((((("a" or "b" or "c" or "d" or "e" or "f" )  and ("g" and ( not "h") )  )  and "i" )  and "j" )  and ("k" and ( not "l") )  )  and "m" )  and ("n" and ( not "o") )  )  and ("p" and "q" )  )

Domain logical expression index: "a"

Referential boundary: The target chemical should be classified as Anilines (Acute toxicity) AND Dianilines by US-EPA New Chemical Categories

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as Radical AND Radical >> Radical mechanism via ROS formation (indirect) AND Radical >> Radical mechanism via ROS formation (indirect) >> Single-Ring Substituted Primary Aromatic Amines AND SN1 AND SN1 >> Nucleophilic attack after nitrenium ion formation AND SN1 >> Nucleophilic attack after nitrenium ion formation >> Single-Ring Substituted Primary Aromatic Amines by DNA binding by OASIS v.1.4

Domain logical expression index: "c"

Referential boundary: The target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Primary aromatic amine by DNA binding by OECD

Domain logical expression index: "d"

Referential boundary: The target chemical should be classified as Strong binder, NH2 group by Estrogen Receptor Binding

Domain logical expression index: "e"

Referential boundary: The target chemical should be classified as AN2 AND AN2 >> Michael-type addition to quinoid structures  AND AN2 >> Michael-type addition to quinoid structures  >> Substituted Anilines by Protein binding by OASIS v1.4

Domain logical expression index: "f"

Referential boundary: The target chemical should be classified as Anilines (Unhindered) AND Benzyl Alcohols by Aquatic toxicity classification by ECOSAR

Domain logical expression index: "g"

Referential boundary: The target chemical should be classified as Strong binder, NH2 group by Estrogen Receptor Binding

Domain logical expression index: "h"

Referential boundary: The target chemical should be classified as Moderate binder, NH2 group OR Non binder, impaired OH or NH2 group OR Non binder, MW>500 OR Non binder, non cyclic structure OR Non binder, without OH or NH2 group OR Strong binder, OH group OR Weak binder, NH2 group OR Weak binder, OH group by Estrogen Receptor Binding

Domain logical expression index: "i"

Referential boundary: The target chemical should be classified as Class 5 (Not possible to classify according to these rules) by Acute aquatic toxicity classification by Verhaar (Modified) ONLY

Domain logical expression index: "j"

Referential boundary: The target chemical should be classified as Bioavailable by Lipinski Rule Oasis ONLY

Domain logical expression index: "k"

Referential boundary: The target chemical should be classified as Non-Metals by Groups of elements

Domain logical expression index: "l"

Referential boundary: The target chemical should be classified as Alkali Earth OR Halogens by Groups of elements

Domain logical expression index: "m"

Similarity boundary:Target: Nc1ccc(C(O)(c2ccc(N)cc2)c2ccc(N)cc2)cc1
Threshold=30%,
Dice(Atom centered fragments)
Atom type; Count H attached; Hybridization

Domain logical expression index: "n"

Referential boundary: The target chemical should be classified as Oxyphenistain (Hepatotoxicity) Alert by Repeated dose (HESS)

Domain logical expression index: "o"

Referential boundary: The target chemical should be classified as Benzene/ Naphthalene sulfonic acids (Less susceptible) Rank C by Repeated dose (HESS)

Domain logical expression index: "p"

Parametric boundary:The target chemical should have a value of log Kow which is >= 0.0765

Domain logical expression index: "q"

Parametric boundary:The target chemical should have a value of log Kow which is <= 3.56

Interpretation of results:

other: not irritating

Conclusions:

The substance 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) was estimated to be not irritating to the skin of New Zealand White rabbits. Based on the estimated result 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) can be considered to be not irritating to skin.

Executive summary:

The dermal irritation potential of 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) was estimated using OECD QSAR toolbox version 3.4 with logPow as the primary descriptor. The substance 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) was estimated to be not irritating to the skin of New Zealand White rabbits. Based on the estimated result 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) can be considered to be not irritating to skin and can be classified under the category ˋ Not Classified’ as per CLP regulation.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records

Reference

Endpoint:

eye irritation: in vivo

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification

Justification for type of information:

Data is from OECD QSAR toolbox version 3.4 and QMRF report has been attached

Qualifier:

according to guideline

Guideline:

other: estimated data

Principles of method if other than guideline:

Prediction is done using QSAR Toolbox version 3.4

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): 4,4',4''-Triaminotrityl alcohol
- Molecular formula: C19H19N3O
- Molecular weight: 305.379 g/mol
- Smiles notation: C(c1ccc(N)cc1)(c1ccc(N)cc1)(c1ccc(N)cc1)O
-InChl: 1S/C19H19N3O/c20-16-7-1-13(2-8-16)19(23,14-3-9-17(21)10-4-14)15-5-11-18(22)12-6-15/h1-12,23H,20-22H2
- Substance type: Organic
- Physical state: Solid

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

No data available

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

No data available

Duration of treatment / exposure:

72 hours

Observation period (in vivo):

72 hour

Duration of post- treatment incubation (in vitro):

No data available

Number of animals or in vitro replicates:

No data available

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

72 h

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

No eye irritation was observed.

Estimation method: Takes mode value from the 5 nearest neighbours
Domain  logical expression:Result: In Domain

(((((("a" or "b" or "c" or "d" or "e" or "f" )  and ("g" and ( not "h") )  )  and ("i" and ( not "j") )  )  and ("k" and ( not "l") )  )  and "m" )  and ("n" and "o" )  )

Domain logical expression index: "a"

Referential boundary: The target chemical should be classified as Anilines (Acute toxicity) AND Dianilines by US-EPA New Chemical Categories

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as Radical AND Radical >> Radical mechanism via ROS formation (indirect) AND Radical >> Radical mechanism via ROS formation (indirect) >> Single-Ring Substituted Primary Aromatic Amines AND SN1 AND SN1 >> Nucleophilic attack after nitrenium ion formation AND SN1 >> Nucleophilic attack after nitrenium ion formation >> Single-Ring Substituted Primary Aromatic Amines by DNA binding by OASIS v.1.4

Domain logical expression index: "c"

Referential boundary: The target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Primary aromatic amine by DNA binding by OECD

Domain logical expression index: "d"

Referential boundary: The target chemical should be classified as Strong binder, NH2 group by Estrogen Receptor Binding

Domain logical expression index: "e"

Referential boundary: The target chemical should be classified as AN2 AND AN2 >> Michael-type addition to quinoid structures  AND AN2 >> Michael-type addition to quinoid structures  >> Substituted Anilines by Protein binding by OASIS v1.4

Domain logical expression index: "f"

Referential boundary: The target chemical should be classified as Anilines (Unhindered) AND Benzyl Alcohols by Aquatic toxicity classification by ECOSAR

Domain logical expression index: "g"

Referential boundary: The target chemical should be classified as Radical AND Radical >> Radical mechanism via ROS formation (indirect) AND Radical >> Radical mechanism via ROS formation (indirect) >> Single-Ring Substituted Primary Aromatic Amines AND SN1 AND SN1 >> Nucleophilic attack after nitrenium ion formation AND SN1 >> Nucleophilic attack after nitrenium ion formation >> Single-Ring Substituted Primary Aromatic Amines by DNA binding by OASIS v.1.4

Domain logical expression index: "h"

Referential boundary: The target chemical should be classified as AN2 OR AN2 >>  Michael-type addition, quinoid structures OR AN2 >>  Michael-type addition, quinoid structures >> Quinones and Trihydroxybenzenes OR AN2 >> Schiff base formation OR AN2 >> Schiff base formation >> Polarized Haloalkene Derivatives OR AN2 >> Schiff base formation by aldehyde formed after metabolic activation OR AN2 >> Schiff base formation by aldehyde formed after metabolic activation >> Geminal Polyhaloalkane Derivatives OR AN2 >> Shiff base formation after aldehyde release OR AN2 >> Shiff base formation after aldehyde release >> Specific Acetate Esters OR AN2 >> Thioacylation via nucleophilic addition after cysteine-mediated thioketene formation OR AN2 >> Thioacylation via nucleophilic addition after cysteine-mediated thioketene formation >> Haloalkenes with Electron-Withdrawing Groups OR AN2 >> Thioacylation via nucleophilic addition after cysteine-mediated thioketene formation >> Polarized Haloalkene Derivatives OR No alert found OR Non-covalent interaction OR Non-covalent interaction >> DNA intercalation OR Non-covalent interaction >> DNA intercalation >> Amino Anthraquinones OR Non-covalent interaction >> DNA intercalation >> DNA Intercalators with Carboxamide and Aminoalkylamine Side Chain OR Non-covalent interaction >> DNA intercalation >> Fused-Ring Primary Aromatic Amines OR Non-covalent interaction >> DNA intercalation >> Quinones and Trihydroxybenzenes OR Radical >> Radical mechanism via ROS formation (indirect) >> Amino Anthraquinones OR Radical >> Radical mechanism via ROS formation (indirect) >> Diazenes and Azoxyalkanes OR Radical >> Radical mechanism via ROS formation (indirect) >> Fused-Ring Primary Aromatic Amines OR Radical >> Radical mechanism via ROS formation (indirect) >> Geminal Polyhaloalkane Derivatives OR Radical >> Radical mechanism via ROS formation (indirect) >> Nitroaniline Derivatives OR Radical >> Radical mechanism via ROS formation (indirect) >> Nitroarenes with Other Active Groups OR Radical >> Radical mechanism via ROS formation (indirect) >> Nitrophenols, Nitrophenyl Ethers and Nitrobenzoic Acids OR Radical >> Radical mechanism via ROS formation (indirect) >> Polynitroarenes OR Radical >> Radical mechanism via ROS formation (indirect) >> Quinones and Trihydroxybenzenes OR SN1 >> Carbenium ion formation OR SN1 >> Carbenium ion formation >> Alpha-Haloethers OR SN1 >> Direct nucleophilic attack on diazonium cation (DNA alkylation) OR SN1 >> Direct nucleophilic attack on diazonium cation (DNA alkylation) >> Diazenes and Azoxyalkanes OR SN1 >> Nucleophilic attack after carbenium ion formation OR SN1 >> Nucleophilic attack after carbenium ion formation >> Specific Acetate Esters OR SN1 >> Nucleophilic attack after diazonium or carbenium ion formation OR SN1 >> Nucleophilic attack after diazonium or carbenium ion formation >> Nitroarenes with Other Active Groups OR SN1 >> Nucleophilic attack after metabolic nitrenium ion formation OR SN1 >> Nucleophilic attack after metabolic nitrenium ion formation >> Amino Anthraquinones OR SN1 >> Nucleophilic attack after metabolic nitrenium ion formation >> Fused-Ring Primary Aromatic Amines OR SN1 >> Nucleophilic attack after reduction and nitrenium ion formation OR SN1 >> Nucleophilic attack after reduction and nitrenium ion formation >> Nitroaniline Derivatives OR SN1 >> Nucleophilic attack after reduction and nitrenium ion formation >> Nitroarenes with Other Active Groups OR SN1 >> Nucleophilic attack after reduction and nitrenium ion formation >> Nitrophenols, Nitrophenyl Ethers and Nitrobenzoic Acids OR SN1 >> Nucleophilic attack after reduction and nitrenium ion formation >> Polynitroarenes OR SN2 OR SN2 >> Acylation OR SN2 >> Acylation >> Specific Acetate Esters OR SN2 >> Acylation involving a leaving group after metabolic activation OR SN2 >> Acylation involving a leaving group after metabolic activation >> Geminal Polyhaloalkane Derivatives OR SN2 >> Alkylation, direct acting epoxides and related after P450-mediated metabolic activation OR SN2 >> Alkylation, direct acting epoxides and related after P450-mediated metabolic activation >> Haloalkenes with Electron-Withdrawing Groups OR SN2 >> Alkylation, direct acting epoxides and related after P450-mediated metabolic activation >> Polarized Haloalkene Derivatives OR SN2 >> Nucleophilic substitution at sp3 Carbon atom OR SN2 >> Nucleophilic substitution at sp3 Carbon atom >> Specific Acetate Esters OR SN2 >> Nucleophilic substitution at sp3 carbon atom after thiol (glutathione) conjugation OR SN2 >> Nucleophilic substitution at sp3 carbon atom after thiol (glutathione) conjugation >> Geminal Polyhaloalkane Derivatives OR SN2 >> SN2 at sp3 and activated sp2 carbon atom OR SN2 >> SN2 at sp3 and activated sp2 carbon atom >> Polarized Haloalkene Derivatives OR SN2 >> SN2 at sp3-carbon atom OR SN2 >> SN2 at sp3-carbon atom >> Alpha-Haloethers OR SN2 >> SN2 attack on activated carbon Csp3 or Csp2 OR SN2 >> SN2 attack on activated carbon Csp3 or Csp2 >> Nitroarenes with Other Active Groups by DNA binding by OASIS v.1.4

Domain logical expression index: "i"

Referential boundary: The target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Primary aromatic amine by DNA binding by OECD

Domain logical expression index: "j"

Referential boundary: The target chemical should be classified as Michael addition OR Michael addition >> P450 Mediated Activation to Quinones and Quinone-type Chemicals OR Michael addition >> P450 Mediated Activation to Quinones and Quinone-type Chemicals >> Hydroquinones OR No alert found by DNA binding by OECD

Domain logical expression index: "k"

Referential boundary: The target chemical should be classified as Strong binder, NH2 group by Estrogen Receptor Binding

Domain logical expression index: "l"

Referential boundary: The target chemical should be classified as Moderate binder, NH2 group OR Weak binder, NH2 group OR Weak binder, OH group by Estrogen Receptor Binding

Domain logical expression index: "m"

Similarity boundary:Target: Nc1ccc(C(O)(c2ccc(N)cc2)c2ccc(N)cc2)cc1
Threshold=40%,
Dice(Atom centered fragments)
Atom type; Count H attached; Hybridization

Domain logical expression index: "n"

Parametric boundary:The target chemical should have a value of log Kow which is >= 0.547

Domain logical expression index: "o"

Parametric boundary:The target chemical should have a value of log Kow which is <= 3.28

Interpretation of results:

other: not irritating

Conclusions:

The substance 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) was estimated to be not irritating into the eyes of New Zealand White rabbits. Based on the estimated result 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) can be considered to be not irritating to eye.

Executive summary:

The ocular irritation potential of 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) was estimated using OECD QSAR toolbox version 3.4 with logPow as the primary descriptor. The substance 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) was estimated to be not irritating into the eyes of New Zealand White rabbits. Based on the estimated result 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) can be considered to be not irritating to eye and can be classified under the category ˋ Not Classified’ as per CLP regulation.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Skin irritation:

Various studies has been investigated for the test chemical4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) to observe the potential for dermal irritation to a greater or lesser extent. The studies are based on in vivo experiments in rabbits for target chemical4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) and its structurally similar read across substances4,4'-Methylenedianiline (CAS No: 101-77-9) and 4,4’-oxydianiline (CAS No: 101-80-4).The predicted data using the OECD QSAR toolbox andDanish QSAR databasehas also been compared with the experimental data and summarized as below;

 

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the skin irritation potential was estimated for test chemical4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) .The chemical 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) is estimated to be not irritating to skin of New Zealand White rabbits.

 

According to Danish QSAR database , the skin irritation effects were estimated by using four different models i.e, Battery, Leadscope, SciQSAR and CASE Ultra for 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9). Based on estimation, no skin irritation reactions were observed in rabbits. Therefore, 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) was considered to be not irritating .

 

The NTRL (National Technical Reports Library, 1986) reported the primary skin irritation study ofstructurally similar read across substance4,4'-Methylenedianiline (CAS No: 101-77-9) on eight (4/sex)New Zealand White rabbits according to Draize method. In this test, 500mg of undiluted chemical was applied to the intact and abraded skin of shornshoulders and the hindquartersof each rabbit under occlusive condition for 24 hours. A 2 x 2 cm area of the shorn skin was abraded using a fine hypodermic needle. Injuries were deep enough to disturb the stratum corneum but not sufficiently deep to cause bleeding. The patches were covered by an occlusivepolyethylene which was secured in position by mean of an elastic adhesive bandage (3" Poroplast).Reactions were assessed visually for the degree of erythema and oedema upto 7 days. In one rabbit, the abraded patch had a bruised appearance. This was thought to be due to damage of the dermis during abrasion. Since none of the rabbit showed erythema or edema, the chemical 4,4'-Methylenedianiline (CAS No: 101-77-9) was considered to be not irritating to the New Zealand White rabbits’ skin.

 

The above results were further supported by the dermal irritation study conducted by U.S. Environmental Protection Agency (2007) onstructurally similar read across substance4,4’-oxydianiline (CAS No: 101-80-4) in six albino rabbit to assess the skin irritation potential caused by the chemical. Six albino rabbits were clipped free of hair on the trunk and lateral areas and placed in FDA-type stocks. 500mg (0.5 g) of 4,4'-Oxydianiline was placed on the trunk of the rabbits under a gauze pad. The trunk of each rabbit was then loosely wrapped with rubber sheeting. After 4 hours, the rabbits were removed from the stocks, washed, and reactions were read according to the system of the Federal Hazardous Substances Act. Readings were also made at 24 and 48 hours. No erythema or edema was observed at 4, 24, or 48 hours. Skin corrosion was not observed in any of the animals. Hence the chemical 4,4’-oxydianiline (CAS No: 101-80-4) was considered to be not irritating to the six albino rabbit’s skin.

 

Thus on the basis of available data for thetarget chemical4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) and its structurally similar read across substances4,4'-Methylenedianiline (CAS No: 101-77-9) and 4,4’-oxydianiline (CAS No: 101-80-4),it can be concluded thatchemical 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) is unable to cause skin irritation and considered as not irritating.Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified”.

 

 

Eye irritation:

In different studies,the test chemical4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) has been investigated for potential for ocular irritationto a greater or lesser extent. The studies are based on in vivo experiments in rabbits for target chemical4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9)its structurally similar read across substances 4,4'-Methylenedianiline (CAS No: 101-77-9) and 4,4’-oxydianiline (CAS No: 101-80-4).The predicted data using the OECD QSAR toolbox has also been compared with the experimental data and summarized as below;

 

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the eye irritation potential was estimated for test chemical4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) .The chemical 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) is estimated to be not irritating to eye of New Zealand White rabbits.

 

The NTRL (National Technical Reports Library, 1986) reported an eye irritation study ofstructurally similar read across substance4,4'-Methylenedianiline (CAS No: 101-77-9) on four (2/sex) albino New Zealand White rabbits. In this test, 10-20 mg of undiluted 4,4'-Methylenedianiline was installed into the conjunctival sac of each rabbit . Following instillation, one eye was washed and the other was not. The eyes were observed for 1, 2, 24, 48 and 168 hours. Since no eye irritation was observed, thechemical4,4'-Methylenedianiline (CAS No: 101-77-9) was considered to be not irritating to the New Zealand White rabbits’ eye.

 

The above results were further supported by the experimental study conducted by U.S. Environmental Protection Agency (2007) onstructurally similar read across substance4,4’-oxydianiline (CAS No: 101-80-4).A dose of 10 mg of solid 4,4’-oxydianiline was placed into the right conjunctival sac of each of 2 rabbits (age not specified). After 20 seconds, 1 treated eye was washed with tap water for 1 minute. The treated eye of the other rabbit was not washed. Observations of the cornea, iris, and conjunctiva were made with an ophthalmoscope at 1 and 4 hours, and at 1, 2, and 3 days. Eyes were stained and a slit-lamp biomicroscope was used at examinations after the day of treatment. After treatment with 4,4’-oxydianiline, washed and unwashed rabbit eyes had slight corneal clouding. The unwashed eye also displayed mild conjunctivitis. Both eyes were normal 1 day after treatment. Since the observed effects were cleared within 1 day, the chemical 4,4’-oxydianiline (CAS No: 101-80-4) was considered to be not irritating to the albino rabbit’s eye.

 

Thus on the basis of available data for thetarget chemical4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) and its structurally similar read across substances4,4'-Methylenedianiline (CAS No: 101-77-9) and 4,4’-oxydianiline (CAS No: 101-80-4),it can be concluded thatchemical 4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) is unable to cause eye irritation and considered as not irritating.Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified”.

 

Justification for classification or non-classification

The skin and eye irritation potential of test chemical4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) and its structurally similar read across substanceswere observed in various studies. The results obtained from these studies indicate that the chemical4,4’,4’’-Triaminotrityl alcoholis unlikely to cause skin and eye irritation. Hence4,4’,4’’-Triaminotrityl alcohol (CAS No: 467-62-9) can be classified under the category “Not Classified” for skin and eye as per CLP.