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EC number: 233-566-4
CAS number: 10236-47-2
1. Summary of results.
Mean OD /disc (#)
Mean OD / product
An in vitro skin irritation test of the test item was performed in
a reconstructed human SkinEthic™ RHE epidermis model,
according to OECD TG 439 (GLP study). Three epidermis units were treated
with 16 mg test item for 42 minutes at room temperature. Exposure of the
test item was terminated by rinsing with 25 x 1 mL of DPBS. The
epidermis units were then incubated at 37°C for 41 hours 12 minutes in
an incubator with 5% CO2. The viability of each disk was assessed by
incubating the tissues with MTT, extracting the precipitated formazan
crystals using isopropanol during 2 hours under gentle agitation in the
dark, and measuring the concentration of formazan by determining the OD
at 570 nm, just after dilution of the extracts 1:2 in isopropanol. Under
test conditions, the mean corrected percent viability of the
treated tissues was 66.7 %, versus 1.0% in the positive control (5%
Sodium Dodecyl Sulfate). Therefore, the test item is not irritant to the
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