Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 229-657-3 | CAS number: 6641-64-1
Endpoint summary
Administrative data
Description of key information
Biodegradation in water:
Biodegradability of test chemical 4,5-Dichloro-2-nitroaniline is predicted using OECD QSAR tool box v.3.3 using log Kow as preimary descriptor. The test chemical 4,5-Dichloro-2-nitroaniline showed 0.44 % biodegradation by using BOD parameter and microorganisms as inoculum in 28 days. By this value of percent biodegradation it is concluded that test chemical 4,5-Dichloro-2-nitroaniline is not readily biodegradable.
Biodegradation in soil and sediments:
Estimation Programs Interface (EPI Suite, 2017) prediction model was run to predict the half-life in water and sediment for the test compound 4,5-Dichloro-2-nitroaniline (CAS No: 6641 -64 -1). If released in to the environment, 13.1 % of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of the chemical 4,5-Dichloro-2-nitroaniline in water is estimated to be 60 days (1440 hrs). The half-life (60 days estimated by EPI suite) indicates that the chemical is persistent in water and the exposure risk to aquatic animals is high whereas the half-life period of 4,5-Dichloro-2-nitroaniline in sediment is estimated to be 541.66 days (13000 hrs).Based on this half-life value, it indicates that 4,5-Dichloro-2-nitroaniline is persistent in sediment.
Biodegradation in soil:
The half-life period of 4,5-Dichloro-2-nitroaniline (CAS No: 6641 -64 -1) in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (EPI suite, 2017). If released into the environment, 86.4 % of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of test chemical 4,5-Dichloro-2-nitroaniline in soil is estimated to be 120 days (2880 hrs). Based on this half-life value of test chemical 4,5-Dichloro-2-nitroaniline, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to low.
Additional information
Biodegradation in water:
Predicted data for the target compound 4,5-Dichloro-2-nitroaniline (CAS no. 6641-64-1) and supporting weight of evidence studies for its read across substance were reviewed for the biodegradation end point which are summarized as below.
In a prediction biodegradability of test chemical 4,5-Dichloro-2-nitroaniline is estimated using OECD QSAR tool box v.3.3 using log Kow as primary descriptor. The test chemical 4,5-Dichloro-2-nitroaniline showed 0.44 % biodegradation by using BOD parameter and microorganisms as inoculum in 28 days. By this value of percent biodegradation it is concluded that test chemical 4,5-Dichloro-2-nitroaniline is not readily biodegradable.
Another prediction done by using Estimation Programs Interface Suite (EPI suite, 2017) to estimate the biodegradation potential of the test chemical 4,5-Dichloro-2-nitroaniline (CAS No: 6641-64-1) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical 4,5-Dichloro-2-nitroaniline is not readily biodegradable.
In a supporting weight of evidence study from authoritative database (J Check, 2017) the read across chemical 4-Chlor-2-nitroanilin (CAS no. 89-63-4) was subjected to biodegradation test by taking sludge as inoculums at 30 mg/L concentration. Biodegradation was analyzed by using three parameters that are BOD, TOC and GC. The initial concentration of read across chemical was 100 mg/L and study design is of standard type. After 2 weeks read across chemical showed 0 % degradation by all three parameters BOD, TOC and GC so it is concluded that read across chemical 4-Chlor-2-nitroanilin is not readily biodegradable.
In another supporting weight of evidence study from authoritative database (J Check, 2017) read across chemical 1,2-Dichloro-4-nitrobenzene (CAS no. 99-54-7) was subjected to biodegradation test by taking sludge as inoculums at 30 mg/L concentration. Biodegradation was analyzed by using three parameters that are BOD, TOC and GC. The initial concentration of read across chemical was 100 mg/L and study design is of standard type. After 4 weeks (28 days) read across chemical1,2-Dichloro-4-nitrobenzene showed 0.0 % degradation by BOD and TOC parameter and 7 % degradation by GC parameter in 28 days so it is concluded that read across chemical1,2-Dichloro-4-nitrobenzene is not readily biodegradable.
On the basis of above results for target chemical 4,5-Dichloro-2-nitroaniline (CAS no. 6641-64-1) (from OECD QSAR tool box v3.4 and EPI suite, 2017) and for its read across substance (from authoritative database), it is concluded that the test substance 4,5-Dichloro-2-nitroaniline is not readily biodegradable in nature.
Biodegradation in soil and sediments:
Estimation Programs Interface (EPI Suite, 2017) prediction model was run to predict the half-life in water and sediment for the test compound 4,5-Dichloro-2-nitroaniline (CAS No: 6641 -64 -1). If released in to the environment, 13.1 % of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of the chemical 4,5-Dichloro-2-nitroaniline in water is estimated to be 60 days (1440 hrs). The half-life (60 days estimated by EPI suite) indicates that the chemical is persistent in water and the exposure risk to aquatic animals is high whereas the half-life period of 4,5-Dichloro-2-nitroaniline in sediment is estimated to be 541.66 days (13000 hrs).Based on this half-life value, it indicates that 4,5-Dichloro-2-nitroaniline is persistent in sediment.
Biodegradation in soil:
The half-life period of4,5-Dichloro-2-nitroaniline(CAS No: 6641 -64 -1) in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (EPI suite, 2017). If released into the environment, 86.4 % of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of test chemical4,5-Dichloro-2-nitroanilinein soil is estimated to be 120 days (2880 hrs). Based on this half-life value of test chemical4,5-Dichloro-2-nitroaniline, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to low.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
