Registration Dossier
Registration Dossier
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EC number: 201-368-7 | CAS number: 81-64-1
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- Principles of method if other than guideline:
- A chromosomal aberration test in CHL/IU Cells was carried out in doses up to 2.5 mg/ml.
- GLP compliance:
- yes
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- 1,4-dihydroxyanthraquinone
- EC Number:
- 201-368-7
- EC Name:
- 1,4-dihydroxyanthraquinone
- Cas Number:
- 81-64-1
- Molecular formula:
- C14H8O4
- IUPAC Name:
- 1,4-dihydroxy-9,10-dihydroanthracene-9,10-dione
- Details on test material:
- Purity: 98.1%
supplier: Wako Jyunyaku Kogyo Co., Ltd.
Constituent 1
Method
- Target gene:
- no data
Species / strain
- Species / strain / cell type:
- mammalian cell line, other: CHL/IU cells
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- short treatment: 0.31, 0.63, 1.3, 2.5 mg/ml
continuous treatment: 0.0013, 0.0025, 0.0050, 0.010 mg/ml
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- mitomycin C
Results and discussion
Test results
- Key result
- Species / strain:
- mammalian cell line, other: CHL/IU cells
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Quinizarine was positive in this CHL/IU test with and without metabolic activation.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: positive
- Executive summary:
A chromosomal aberration test in CHL/IU cells was carried out in doses up to 2.5 mg/ml. Quinizarine was positive in this CHL/IU test with and without metabolic activation.
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