1,4-dimethoxybenzene

Specific investigations: other studies

Administrative data

Endpoint:

cytotoxicity

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well described

Data source

Reference

Reference Type:

publication

Title:

Development of an in vitro primary screen for skin depigmentation and antimelanoma agents

Author:

Dooley T.P. , Gadwood R.C. , Kilgore K. and Thomasco L.M.

Year:

1994

Bibliographic source:

Skin Pharmacology, 7(4), 188-200.

Materials and methods

Principles of method if other than guideline:

In vitro cell culture system with spontaneously immortalized murine melanocyte lines that growth rapidly in culture and produces copious amounts
of melanine, was treated with PDMB and similar compounds. This method evaluate the ability of compound to inhibiting melanogenesis or to cause
melanocyte/melanoma cell death, by measuring the spectrophotometric means of melanin after 5 days of treatment.

GLP compliance:

not specified

Type of method:

in vitro

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dimethoxybenzene

EC Number:

205-771-9

EC Name:

1,4-dimethoxybenzene

Cas Number:

150-78-7

Molecular formula:

C8H10O2

IUPAC Name:

1,4-dimethoxybenzene

Details on test material:

The substance is commercially product, provided by Aldrich Chemical Co., Milwaukee, Wisconsin.

Test animals

Species:

mouse

Strain:

C57BL

Sex:

not specified

Details on test animals or test system and environmental conditions:

In vitro test:
a  C57BL/6 mouse-derived spontaneously immortalized melanocyte cell line

Administration / exposure

Route of administration:

other: in vitro

Analytical verification of doses or concentrations:

no

Duration of treatment / exposure:

See details below

Frequency of treatment:

See details below

Post exposure period:

See details below

No. of animals per sex per dose:

Not applicable

Results and discussion

Details on results:

The Mel-Ab melanocyte cell line was exposed to test material for 5 days.  Vehicle was 50% propylene glycol, 30% ethanol and 20% water. Melanin 
content and cell survival were measured Comments All materials were  tested at 0.01-1000 ug/ml. The inhibitory concentration of 50% values for 
PDMB on melanocyte cell  line (Mel-Ab) is 280 µg/mL. This IC50 value represents the concentration  at which melanine biosynthesis has decreased 
by one half, relative to  vehicle control treated cells. That means a reduction in either  depigmentation directly on cytotoxic or cytostatic effect of 
compounds on  total cell number. An IC50 exceeding or equal to 100 µg/mL was considered  as inactive. The PDMB was by consequence considered as inactive for the depigmentation  of this melanocyte cell lines (Mel-Ab) or for the cytotoxic or cytostatic  effect. Comparing this result with the 
active hydroquinone effects, the authors  concluded that the decreased oxidation potential of di-methoxy analogs  relative to di-hydroxy 
compounds accounts for the reduction of  cytotoxicity.