4-chloro-1-methylpiperidinium chloride

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

data is from peer reviewed journals

Data source

Reference

Reference Type:

publication

Title:

Range-Finding Toxicity Data: List VI

Author:

Henry F. Smyth Jr., Charles P. Carpenter, Carrol S. Well, Urbano C. Pozzani & Jean A. Striegel

Year:

1962

Bibliographic source:

American Industrial Hygiene Association Journal, 1962, 23:2, 95-107

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Primary dermal irritation study was carried out on rabbits to assess the irritation potential of 1-methylpiperazine

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1-methylpiperazine

EC Number:

203-639-5

EC Name:

1-methylpiperazine

Cas Number:

109-01-3

Molecular formula:

C5H12N2

IUPAC Name:

1-methylpiperazine

Test material form:

liquid

Details on test material:

Name of test material (as cited in study report): 1-methyl piperazine
Molecular formula : C5H12N2
Molecular weight : 100.1638 g/mol
Substance Type: Organic
Physical State: Liquid

Specific details on test material used for the study:

Name of test material (as cited in study report): 1-methyl piperazine
Molecular formula : C5H12N2
Molecular weight : 100.1638 g/mol
Substance Type: Organic
Physical State: Liquid

Test animals

Species:

rabbit

Strain:

other: albino

Details on test animals or test system and environmental conditions:

no data available

Test system

Type of coverage:

open

Preparation of test site:

clipped

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

0.01 ml of undiluted sample or of solutions in water, acetone, propylene glycol

Duration of treatment / exposure:

24 hours

Observation period:

24 hours

Number of animals:

5

Details on study design:

TEST SITE
Area of exposure: closely clipped, intact abdominal skin
% coverage: No data
Type of wrap if used: no wrap used

REMOVAL OF TEST SUBSTANCE
Washing (if done): no data
Time after start of exposure: no data

SCORING SYSTEM:
Primary skin irritation on rabbits was recorded in a 10-grade ordinal series. Grade 1 indicates no irritation and Grade 2 the least visible capillary injection from the undiluted chemical. Grade 6 indicates necrosis when undiluted and Grade 10 indicates necrosis from a 0.01% solution

Results and discussion

In vitro

Other effects / acceptance of results:

no data available

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

24 h

Score:

6

Max. score:

10

Reversibility:

not specified

Remarks on result:

probability of moderate irritation

Irritant / corrosive response data:

Primary Irritation score after 24 hours for 1-methylpiperazine was Grade 6

Any other information on results incl. tables

 Table: Results of the irritation study

Material tested	Irritation on uncovered rabbit belly
1 -methylpiperazine	6

                                                    

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Conclusions:

Acute dermal irritation study was carried out to assess the irritation potential of 1-methyl piperazine.
Primary Irritation score after 24 hours for 1-methylpiperazine was Grade 6.
Based on this grade, 1-methyl piperazine can be considered moderately irritating to rabbit skin.

Executive summary:

Acute dermal irritation study was carried out to assess the irritation potential of 1-methylpiperazine.

Primary skin irritation on rabbits was recorded in a 10-grade ordinal series and was based upon the severest reaction that develops on the clipped abdominal skin of each of five albino rabbits within 24 hours of the uncovered application of 0.01 ml of undiluted sample or of solutions in water, propylene glycol, or acetone.

Grade 1 indicates no irritation and Grade 2 the least visible capillary injection from the undiluted chemical. Grade 6 indicates necrosis when undiluted and Grade 10 indicates necrosis from a 0.01% solution

Primary Irritation score after 24 hours for 1 -methyl piperazine was Grade 6.

Based on this grade,1-methylpiperazine can be considered moderately irritating to rabbit skin.