1,1'-[(6-phenyl-1,3,5-triazine-2,4-diyl)diimino]bis[3-acetyl-4-aminoanthraquinone]

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1986

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

not specified

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,1'-[(6-phenyl-1,3,5-triazine-2,4-diyl)diimino]bis[3-acetyl-4-aminoanthraquinone]

EC Number:

250-961-7

EC Name:

1,1'-[(6-phenyl-1,3,5-triazine-2,4-diyl)diimino]bis[3-acetyl-4-aminoanthraquinone]

Cas Number:

32220-82-9

Molecular formula:

C41H27N7O6

IUPAC Name:

1,1'-[(6-phenyl-1,3,5-triazine-2,4-diyl)diimino]bis[3-acetyl-4-aminoanthraquinone]

Test material form:

other: mikrodispers

Details on test material:

Cibanon Blau 83962 Mikrodispers

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

activated sludge

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Post exposure observation period:

No data

Test conditions

Hardness:

No data

Test temperature:

No data

pH:

No data

Dissolved oxygen:

No data

Salinity:

Not applicable

Nominal and measured concentrations:

- Nominal concentrations: 10, 32 and 100 mg/L

Details on test conditions:

No data

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Results with reference substance (positive control):

IC 50 (3 hours) = 21 mg/L

Reported statistics and error estimates:

No data

Any other information on results incl. tables

Results test item:

	3 hours
Test item in mg/L	O2 -consumption (mg/Liter/min)	Inhibition (%)
100	1.0	0.0
32	1.0	0.0
10	1.0	0.0
Control 1	0.83
Control 2	0.89

Results reference substance:

	3 hours
3,5 -Dichlorophenol (mg/L)	O2 -consumption (mg/Liter/min)	Inhibition (%)
100	0.06	93.1
32	0.21	75.6
10	1.03	0

Applicant's summary and conclusion

Conclusions:

IC50 (3 hours ) of the test item is found to be greater than 100 mg/L.

Executive summary:

Introduction

A study was performed to assess the effect of the test item on the respiration of activated sewage sludge. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2010) No. 209 "Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation)".

 

 

Methods…….

Activated sewage sludge was exposed to an aqueous solution of the test item at concentrations of 10, 32 and 100 mg/L for a period of 3 hours.

 

The rate of respiration was determined after 3 hours contact time and compared to data for the control and a reference item, 3,5-dichlorophenol.

 

 

Results…….

The effect of the test item on the respiration of activated sewage sludge gave a 3‑hour EC₅₀value of greater than 100 mg/L. 

 The reference item gave a 3-hour EC₅₀value of 21 mg/L.

Conclusion

The effect of the test item on the respiration of activated sewage sludge gave a 3‑hour EC₅₀value of greater than 100 mg/L. 

 The reference item gave a 3-hour EC₅₀value of 21 mg/L.