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Toxicological information

Dermal absorption

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Administrative data

Endpoint:
dermal absorption in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Recent study, well reported in open literature.

Data source

Reference
Reference Type:
publication
Title:
Potential of the octanol-water partition coefficient (logP) to predict the dermal penetration behaviour of amphiphilic compounds in aqueous solutions.
Author:
Korinth G, Wellner T, Schaller KH, Drexler H
Year:
2012
Bibliographic source:
Toxicology Letters 125, 49-53

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 428 (Skin Absorption: In Vitro Method)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Source: Hydro (formerly Vereinigte Aluminium Werke, Grevenbroich, Germany)
Purity: >98%.

Administration / exposure

Vehicle:
other: 50% in 0.9% NaCl in water.
Details on in vitro test system (if applicable):
SKIN PREPARATION
- Source of skin: Local hospital
- Ethical approval if human skin: no information.
- Type of skin: Human abdomen removed following reductive plastic surgery.
- Preparative technique: The skin was separated from subcutaneous fat tissue, wrapped in aluminium foil, and stored in plastic bags. Prior to experiments, the skin was thawed at room temperature.
- Thickness of skin (in mm): 0.9mm
- Membrane integrity check: The integrity of skin membranes was controlled visually and by measurement of skin surface temperatures using a digital quick response precision thermometer with a surface measuring probe.
- Storage conditions: −20C for up to 8 months.

PRINCIPLES OF ASSAY
- Diffusion cell: 0.64cm2
- Receptor fluid: 0.9% NaCl solution in water, stirred continuously.
- Solubility of test substance in receptor fluid: fully miscible.
- Static system: yes
- Test temperature: 37C
- Occlusion: yes
- Reference substance(s):
- Other: Substance was applied as neat liquids or as 50% (v/v) aqueous solutions under infinite dose. The receptor fluid was stirred continuously, and samples were collected 8 h after compound exposure. Each sampled volume was immediately replaced with fresh receptor fluid. Samples were deep frozen and analysed within several days. Detection was was accomplished by solid-phase extraction and subsequent GC/FID analysis.

Results and discussion

Percutaneous absorptionopen allclose all
Remarks on result:
other: Average flux
Remarks:
neat compound: 1389±400ug/cm2/hr
Remarks on result:
other: Average flux
Remarks:
50% solution: 2133±101ug/cm2/hr

Applicant's summary and conclusion

Executive summary:

In a study designed to generate data to confirm or disprove a hypothesis that rates of dermal penetration can be predicted from the octanol water partion coefficient, the rate of 1 -ethoxyypropano-2 -ol penetration in vitro through human skin was determined. The rate of flux of neat substance was quite high at ~1400 ±400ug/cm2/hr (similar to the level measured for ethanol) and increased by around 50% to around 2100±100ug/cm2/hr in the presence of water (50% aqueous solution).