Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 478-250-9 | CAS number: -
Adsorption / desorption
Administrative data
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study report available
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- soil
Test material
- Reference substance name:
- -
- EC Number:
- 478-250-9
- EC Name:
- -
- Cas Number:
- 62965-37-1
- Molecular formula:
- C26H42N2O4
- IUPAC Name:
- (2S)-2-{[(benzyloxy)carbonyl]amino}-3,3-dimethylbutanoic acid; N-cyclohexylcyclohexanamine
Constituent 1
Study design
HPLC method
- Details on study design: HPLC method:
- HPLC CONDITIONS
Instrument: Hewlett Packard 1050 Liquid Chromatograph
Column: Hypersil CPS (25 cm x 4.6 mm internal diameter)
Column temperature: 25°C
Mobile phase composition: Acetonitrile:buffer solution (55:45 v/v), where phosphate and borate buffer solutions were employed for the pH 2.5 and
pH 9 tests respectively.
Flow rate: 1.0 ml/min
Injection volume: 20 µl
Detector: UV set at 210 nm
Results and discussion
Adsorption coefficientopen allclose all
- Type:
- log Koc
- Value:
- 2.2
- Type:
- log Koc
- Value:
- < 1.3
- Remarks on result:
- other: pH 2.5
- Type:
- log Koc
- Value:
- < 1.3
- Remarks on result:
- other: pH 9
Results: HPLC method
- Details on results (HPLC method):
- - Retention times of reference substances used for calibration: six reference substances were used to have log10Koc values between 1.3 and 5.6. An internal standard was used to determine the deadline of HPLC system.
- Calibration curve log10 k vs. log10 Koc runs at pH 2.5. The mean calibration curve was used the interpret the results.
- Average retention data for test substance: 4.015 minutes
Results: Batch equilibrium or other method
- Transformation products:
- no
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance was found to be have a log10Koc values of 2.2 and less than 1.3 at pH 2.5 and pH 9 respectively
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
