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Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
26.05.2017 to 29.05.2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Experimental test result performed using standard test guideline
Justification for type of information:
Experimental test result performed using standard test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Principles of method if other than guideline:
Aim of this study was to evaluate the nature of chemical when comes in contact with the test organism. Test was conducted according to the OECD guideline 201.
GLP compliance:
not specified
Specific details on test material used for the study:
- IUPAC name: 1-bromo-3-methoxybenzene
- Name of test material (as cited in study report): 1-bromo-3-methoxybenzene
- Molecular formula (if other than submission substance): C7H7BrO
- Molecular weight (if other than submission substance): 187.035 g/mol
- Smiles notation (if other than submission substance): c1(cc(ccc1)Br)OC
- InChI: 1S/C7H7BrO/c1-9-7-4-2-3-6(8)5-7/h2-5H,1H3
- Substance type: Organic
- Physical state: Liquid
Analytical monitoring:
not specified
Vehicle:
not specified
Details on test solutions:
The stock solution 100 mg/l was prepared by dissolving colourless liquid in OECD growth medium. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with OECD growth medium. Stock solution was kept in ultrasonic bath for 20 min and then inoculated with algae.
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name:
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available

ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Test temperature:
23±2°C
pH:
Test at highest concentration: 7.9 did not change during test
Control: 8.1 to 7.9
Nominal and measured concentrations:
0, 16, 24, 36, 53 and 80 mg/l nominal concentrations were used
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 15ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (K2Cr2O7)
Key result
Duration:
72 h
Dose descriptor:
other: ErC50
Effect conc.:
55.6 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95 % CI was 52-59.4 mg/l
Results with reference substance (positive control):
- Results with reference substance valid
- EC50: 0.75 mg/L (24 hours)
Reported statistics and error estimates:
EC50 was calculated using non linear regression by the software Prism 4.0
Validity criteria fulfilled:
yes
Conclusions:
Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of chemical the ErC50 was determine to be 55.6 mg/l.
Executive summary:

Aim of this study was to evaluate the nature of test chemical, when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 100 mg/l was prepared by dissolving colourless liquid in OECD growth medium. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with OECD growth medium. Stock solution was kept in ultrasonic bath for 20 min and then inoculated with algae.

With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.

The median effective concentration (ERC50) for the test substance 1-bromo-3-methoxybenzene, in algae was determined to be 55.6 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the ErC50 value the substance is likely to be hazardous to aquatic algae and can be be classified as aquatic chronic 3 category as per the CLP classification criteria.

Description of key information

Aim of this study was to evaluate the nature of test chemical, when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 100 mg/l was prepared by dissolving colourless liquid in OECD growth medium. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with OECD growth medium. Stock solution was kept in ultrasonic bath for 20 min and then inoculated with algae. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The median effective concentration (ERC50) for the test substance 1-bromo-3-methoxybenzene, in algae was determined to be 55.6 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the ErC50 value the substance is likely to be hazardous to aquatic algae and can be be classified as aquatic chronic 3 category as per the CLP classification criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:
55.6 mg/L

Additional information

Aim of this study was to evaluate the nature of test chemical, when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 100 mg/l was prepared by dissolving colourless liquid in OECD growth medium. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with OECD growth medium. Stock solution was kept in ultrasonic bath for 20 min and then inoculated with algae. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The median effective concentration (ERC50) for the test substance 1-bromo-3-methoxybenzene, in algae was determined to be 55.6 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the ErC50 value the substance is likely to be hazardous to aquatic algae and can be be classified as aquatic chronic 3 category as per the CLP classification criteria.