4-nitro-o-toluidine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

Acute fish toxicity data of test substance 2-Methyl-4-nitrobenzenamine for exposure period of 96 hrs. on the basis of mortality effects.

GLP compliance:

not specified

Specific details on test material used for the study:

- IUPAC name 2-methyl-4-nitroaniline
- Name of test material: 4-nitro-o-toluidine
- Molecular formula: C7H8N2O2
- Molecular weight: 152.152 g/mol
- Physical state: Solid
- SMILES: c1(cc(c(N)cc1)C)[N+](=O)[O-]
- InChI: 1S/C7H8N2O2/c1-5-4-6(9(10)11)2-3-7(5)8/h2-4H,8H2,1H3

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name:Fathead Minnow)
- LIFE STAGE:Juvenile(s)
- Length at study initiation (length definition, mean, range and SD):2.4 CM
- Weight at study initiation (mean and range, SD):0.28 G

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

29.6 (12.0- 43.0)mg/L CaCO3

Test temperature:

20.3 (19.5-22.0) deg.C

pH:

7.6 (6.0- 9.2)

Dissolved oxygen:

7.2 (2.8-9.4) mg/L

Salinity:

ALKALINITY: 31.0 (15 - 60) mg/L CaCO3

Conductivity:

66.8 uS

Nominal and measured concentrations:

No data

Details on test conditions:

No data

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

86.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not specified

Conclusions:

In acute fish toxicity study conducted for 2-methyl-4-nitroaniline, the median ethal concentration (LC50) on the basis of mortality effect for Pimephales promelas (Fathead Minnow) was observed to be 86.7 mg/l for exposure period of 96 hrs.

Executive summary:

Acute fish toxicity was conducted for 2-methyl-4-nitroaniline for exposure period of 96 hrs. Test organism was used as Pimephales promelas (Fathead Minnow)in static freshwater with test temp. 20.3 (19.5-22.0) deg.C ,hardness 29.6 (12.0- 43.0)mg/L CaCO3 ,pH7.6 (6.0- 9.2) , ALKALINITY:31.0 (15 - 60) mg/L CaCO3 ,conductivity 66.8 uS and dissolved oxygen 7.2 (2.8-9.4) mg/L. The median ethal concentration (LC50) on the basis of mortality effect for Pimephales promelas (Fathead Minnow) was observed to be 86.7 mg/l for exposure period of 96 hrs. Based on the value, the chemical was considered to be toxic to fish and can be considered to be classified in chronic category 3 as per the CLP regulations.

Description of key information

Acute fish toxicity was conducted for 2-methyl-4-nitroaniline. The median ethal concentration (LC50) on the basis of mortality effect for Pimephales promelas (Fathead Minnow) was observed to be 86.7 mg/l for exposure period of 96 hrs.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

86.7 mg/L

Additional information

Acute fish toxicity was conducted for 2-methyl-4-nitroaniline for exposure period of 96 hrs. Test organism was used as Pimephales promelas (Fathead Minnow)in static freshwater with test temp. 20.3 (19.5-22.0) deg.C ,hardness 29.6 (12.0- 43.0)mg/L CaCO3 ,pH7.6 (6.0- 9.2) , ALKALINITY:31.0 (15 - 60) mg/L CaCO3 ,conductivity 66.8 uS and dissolved oxygen 7.2 (2.8-9.4) mg/L. The median ethal concentration (LC50) on the basis of mortality effect for Pimephales promelas (Fathead Minnow) was observed to be 86.7 mg/l for exposure period of 96 hrs. Based on the value, the chemical was considered to be toxic to fish and can be considered to be classified in chronic category 3 as per the CLP regulation.