2,2-bis(allyloxymethyl)butan-1-ol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

March 1 - 6, 2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP compliant study conducted according the appropriate test guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-allyloxymethyl-2-ethylpropanediol

EC Number:

211-662-7

EC Name:

2-allyloxymethyl-2-ethylpropanediol

Cas Number:

682-11-1

Molecular formula:

C9H18O3

IUPAC Name:

2-[(allyloxy)methyl]-2-methylbutane-1,1-diol

Constituent 2

Reference substance name:

trimethylolpropane monoallyl ether (TMPME)

IUPAC Name:

trimethylolpropane monoallyl ether (TMPME)

Details on test material:

Concentration of TMPME - 99.0%.
Lot number - 3852802
Appearance - clear, colourless liquid
Density - 1010 kg/m3

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
TMPME is being used as a surrogate for TMPDE.

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Portions of the test solution was sampled before the test and after 24 hours for analysis in the Sponsor's Laboratory. The analytical details and results have not been supplied.

Test solutions

Vehicle:

no

Details on test solutions:

24 hours prior to each exposure period 50 g of TMPME was dissolved in one litre of aerated dilution water and stirred for 24 hours. The water phase was used as the stock solution.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

No details have been reported. The fish species tested is not explicity stated but can be inferred from the statement that "the dilution water should allow survival of zebra fish for at least 96 hours".

Fish were placed in quarantine for 14 days prior to use in the study.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not applicable

Test conditions

Hardness:

Dilution water had a hardness in the range of 250 ± 25 mg calcium carbonate/L.

Test temperature:

The range over the course of the study was 23 ± 1°C

pH:

The range over the course of the study was 7.4 - 8.0

Dissolved oxygen:

The range over the course of the study was 73 - 100 % air saturation

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal exposure concentrations: 0 (xcontrol), 200, 400, 800, 1600 and 3200 mg/L

Details on test conditions:

No details

Reference substance (positive control):

yes

Remarks:

Disodium heptachromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Details on results:

Visible abnormalities (e.g. darker pigmentation, loss of equilibrium, disturbance in swimming behavior or increased respiration) were observed in all of the fish in the 800 mg/L treatment.

Results with reference substance (positive control):

The 24 hour LC50 of disodium heptachromate fulfilled the acceptance cirterion of 200 - 400 mg/L (S 028162).

Reported statistics and error estimates:

Not reported.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Fish mortality during the study is presented below:

Nominal exposure conc. (mg/L)	Cumulative mortality (seven fish per concentration)					% mortality at 96 hours
	3 hours	24 hours	48 hours	72 hours	96 hours
Control	0	0	0	0	0	0
200	0	0	0	0	0	0
400	0	0	0	0	0	0
800	0	0	0	0	0	0
1600	6	7	7	7	7	100
3200	7	7	7	7	7	100

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Zebra fish were exposed to TMPME at nominal concentrations of 200, 400, 800, 1600 and 3200 mg/L in a semi-static 96 -hour acute toxicity test according to OECD 203 guidelines. The test design included an untreated control comprising of dilution water only. The 96-hour LC50 determined was 1200 mg TMPME/L (based nominal exposure concentrations). The NOEC (no-observed effect concentration) was 400 mg TMPME/L.

Executive summary:

Zebra fish were exposed to TMPME at nominal concentrations of 200, 400, 800, 1600 and 3200 mg/L in a semi-static 96 -hour acute toxicity test according to OECD 203 guidelines. The test design included an untreated control comprising of dilution water only. The 96-hour LC50 determined was 1200 mg TMPME/L (based nominal exposure concentrations). The NOEC (no-observed effect concentration) was 400 mg TMPME/L.