2,4-dioxo-1,3-diazetidine-1,3-bis(methyl-m-phenylene) diisocyanate

Short-term toxicity to fish

Description of key information

No toxic effects against fish were observed under exposure conditions (96 h-LL50  > 100 mg/L). 


  

The results are expressed in terms of Lethal Loading (LL) (Spoo.Klöppel, 2015).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

"Should read: > 100 mg/L"

A study was performed to assess the acute toxicity to Danio rerio under static conditions. The study was conducted in accordance with Council Regulation (EC) No 440/2008, Method C.1 'Acute toxicity for Fish' (2008) which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992). An acute daphnia toxicity test and an algal growth inhibition test preceded the fish test. They provided information about the loading which was used in the fish test. The following loading of the test item were tested in the other tests. Acute daphnia toxicity test: 100 mg/L, Algal growth inhibition test: 6.25, 12.5, 25, 50 and 100 mg/L. Because of the anticipated low toxicity of the test item, a group of seven fish were exposed in the main test to a limit lethal loading of nominally 100 mg/L for a period of 96 hours. As no toxic effects against fish were observed, no statistical analysis was required to determine the LL 50. Additionally any abnormal behaviour or appearance of the fish was reported every 24 hours. The main test was conducted as static test with the test medium unchanged throughout the duration of the test. During the test a temperature range of 20-24 °C was maintained in the test vessels, with a maximum temperature fluctuation of +/- 1 °C in the test. The temperature, the pH and the oxygen values were measured at the beginning of the test and every 24 hours thereafter. The validity criteria of the test were met. Auxiliaries used to prepare the test media were an ultrasonic bath, a magnetic stirrer and a folded filter. Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, and 96 hours of exposure. After 96 hours no toxic effects against fish were observed at a lethal loading of 100 mg/L. The results are expressed in terms of Lethal Loading (LL) as no specific analytical monitoring is possible. Analytical monitoring was performed via DOC. This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute Fish study.