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Key value for chemical safety assessment

Genetic toxicity in vitro

Description of key information

3'-hydroxyacetophenone is not a gene mutant in vitro.

Link to relevant study records
Reference
Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed publication
Qualifier:
according to
Guideline:
other: refer below principle
Principles of method if other than guideline:
Gene mutation toxicity study was performed to determine the mutagenic nature of the test compound 3'-hydroxyacetophenone
GLP compliance:
not specified
Type of assay:
bacterial gene mutation assay
Target gene:
Histidine
Species / strain / cell type:
other: Salmonella typhimurium LT-2 strains TA 98 and TA 100
Details on mammalian cell type (if applicable):
Not applicable
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
Liver fraction (S-9) from Aroclor 1254 or methylcholanthrene induced rats
Test concentrations with justification for top dose:
0 or 3 µmole/plate
Vehicle / solvent:
- Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: No data available
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
yes
Positive control substance:
other: Yes N-methyl-N'-nitro-N-nitrosoguanidin (without metabolic activation) and 2-aminoanthracene (with activation)
Details on test system and experimental conditions:
METHOD OF APPLICATION: Spot test (in agar)

DURATION
- Preincubation period: No data available
- Exposure duration: No data available
- Expression time (cells in growth medium): No data available
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available

SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available

NUMBER OF REPLICATIONS: No data available

NUMBER OF CELLS EVALUATED: No data available

DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available

OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other: No data available

OTHER: No data available
Evaluation criteria:
1. Increase in the number of spontaneous revertants
2. The presence of the rfa-mutation was checked by crystal violet inhibition;
3. The presence of the plasmid pKM 101 in strains TA 98 and TA 100 was checked by resistance to ampicillin
Statistics:
No data available
Species / strain:
other: Salmonella typhimurium LT-2 strains TA 98, TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
valid
Additional information on results:
No data available
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.
Conclusions:
Interpretation of results :
negative with and without

The test compound 3'-hydroxyacetophenone is not mutagenic in the bacterium Salmonella typhimurium LT-2 strains TA 98, TA 100 with and without S9 metabolic activation system and hence is not likely to classify as gene mutant in vitro.
Executive summary:

Gene mutation toxicity study was performed to determine the mutagenic nature of the test compound 3'-hydroxyacetophenone.

 

The test material was dissolved in dimethsulphoxide (vehicle control) and applied at a concentration of 3 µmole/plate in the spot test performed.

 

3'-hydroxyacetophenone did not induce reversion of mutant strains and hence is not mutagenic in the bacterium Salmonella typhimurium LT-2 strains TA 98, TA 100 with and without S9 metabolic activation system and hence is not likely to classify as gene mutant in vitro.

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (negative)

Additional information

Gene mutation in vitro:

Various peer reviewed publications for the test chemical 3'-hydroxyacetophenone were reviewed to determine its mutagenic nature and are summarized as below

Gene mutation toxicity study was performed by Florins et al (1980) to determine the mutagenic nature of the test compound 3'-hydroxyacetophenone. The test material was dissolved in dimethsulphoxide and applied at a concentration of 3 µmole/plate in the spot test performed. 3'-hydroxyacetophenone did not induce reversion of mutant strains and hence is not mutagenic in the bacterium Salmonella typhimurium LT-2 strains TA 98, TA 100 with and without S9 metabolic activation system and hence is not likely to classify as gene mutant in vitro.

Gene mutation toxicity study was performed by Rapson et al (1980) to determine the mutagenic nature of the test compound 3'-hydroxyacetophenone at dose levels of 0.1, 1, 10, 100 or 1000 µg/plate. The study was performed as part of mutagenicity produced by aqueous chlorination of organic compounds. 3'-hydroxyacetophenone did not induce reversion of mutant strains without chlorination and hence is not mutagenic in the bacterium Salmonella typhimurium TA 100 and hence is not likely to classify as gene mutant in vitro.

Based on the available data summarized, the test chemical 3'-hydroxyacetophenone is not likely to classify as a gene mutant in vitro.

Justification for classification or non-classification

Based on the available data summarized, the test chemical 3'-hydroxyacetophenone is not likely to classify as a gene mutant in vitro.