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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of test chemical 1-(3-hydroxyphenyl)ethanone on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material (as cited in study report): 1-(3-hydroxyphenyl)ethanone
- Molecular formula: C8H8O2
- Molecular weight: 136.149 g/mol
- Smiles: c1(cc(ccc1)O)C(C)=O
- InChl): -1S/C8H8O2/c1-6(9)7-3-2-4-8(10)5-7/h2-5,10H,1H3
-Substance type: organic
- Physical state: solid
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 2.04 cm
- Weight at study initiation (mean and range, SD): average 0.084 g
-Housing: The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
24, 48, 72 and 96 hrs
Hardness:
185 mg of CaCO3
Test temperature:
23 °C
pH:
-------
Dissolved oxygen:
6.3 - 6.7
Nominal and measured concentrations:
100 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Other details not known

Table 1: Visible Symptoms:

Nominal Concentration

(mg/l)

Number of fish

Symptom (cumulated) after

3 h

6 h

24 h

48 h

72 h

96 h

V1

V1

V1

V1

V1

V1

Control

8

NS4

NS4

NS4

NS4

NS4

NS4

100

8

NS4

NS4

NS4

M3

M3

M2

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

E: Exopthalamos

SC: Schooling Effect

Table 2: pH

Nominal Concentration

(mg/l)

 

pH readings after

Number of fish

24 h

48 h

72 h

96 h

V1

V1

V1

V1

Control

8

7.4

7.5

7.6

7.7

100

8

7.8

7.9

8.0

8.1

Table 3: Oxygen content

Nominal Concentration

(mg/l)

 

Oxygen content (mg/L) after

Number of fish

24 h

48 h

72 h

96 h

V1

V1

V1

V1

Control

8

6.7

6.6

6.5

6.4

100

8

6.6

6.5

6.4

6.3

Table 4: Test temperature

Nominal Concentration

(mg/l)

 

Temperature (°C) after

Number of fish

24 h

48 h

72 h

96 h

V1

V1

V1

V1

Control

8

23.9

23.8

24.1

23.8

100

8

23.9

23.8

24.1

23.8

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentration, experimental median lethal Concentrations [LC-50 (96 h)] for 1-(3-hydroxyphenyl)ethanone on Zebra Fish Danio rerio was determine to be 100 mg/l.
Executive summary:

Study was conducted to assess the effect of test chemical 1-(3-hydroxyphenyl)ethanone on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Plastic aquaria were loaded with 8 fishes. A static procedure was used for the study. Test consider to be valid as it fulfilled all the necessary criteria. The mortality in the control was found to be 0 per cent at the end of the test. Constant conditions was maintained throughout the test and, static procedures used. The dissolved oxygen concentration was found to be 78.3 per cent of the air saturation value throughout the test. After 96 hours of exposure to test item 1-(3-hydroxyphenyl)ethanone to limit test concentration 100 m/l, LC50 was determine to be 100 mg/l. Based on the LC50 value, it can be consider that the chemical was toxic, but the chemical was readily biodegradable in water thus on that criteria chemical consider to be nontoxic and not classified as per the CLP classification criteria.

Description of key information

Study was conducted to assess the effect of test chemical 1-(3-hydroxyphenyl)ethanone on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Plastic aquaria were loaded with 8 fishes. A static procedure was used for the study. Test consider to be valid as it fulfilled all the necessary criteria. The mortality in the control was found to be 0 per cent at the end of the test. Constant conditions was maintained throughout the test and, static procedures used. The dissolved oxygen concentration was found to be 78.3 per cent of the air saturation value throughout the test. After 96 hours of exposure to test item 1-(3-hydroxyphenyl)ethanone to limit test concentration 100 m/l, LC50 was determine to be 100 mg/l. Based on the LC50 value, it can be consider that the chemical was toxic, but the chemical was readily biodegradable in water thus on that criteria chemical consider to be nontoxic and not classified as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

Study was conducted to assess the effect of test chemical 1-(3-hydroxyphenyl)ethanone on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Plastic aquaria were loaded with 8 fishes. A static procedure was used for the study. Test consider to be valid as it fulfilled all the necessary criteria. The mortality in the control was found to be 0 per cent at the end of the test. Constant conditions was maintained throughout the test and, static procedures used. The dissolved oxygen concentration was found to be 78.3 per cent of the air saturation value throughout the test. After 96 hours of exposure to test item 1-(3-hydroxyphenyl)ethanone to limit test concentration 100 m/l, LC50 was determine to be 100 mg/l. Based on the LC50 value, it can be consider that the chemical was toxic, but the chemical was readily biodegradable in water thus on that criteria chemical consider to be nontoxic and not classified as per the CLP classification criteria.