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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report date:
2015

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
8-isopropyl-6-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
EC Number:
267-308-7
EC Name:
8-isopropyl-6-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
Cas Number:
67845-30-1
Molecular formula:
C13H20O
IUPAC Name:
8-isopropyl-6-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
Constituent 2
Chemical structure
Reference substance name:
7-isopropyl-5-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
EC Number:
299-795-7
EC Name:
7-isopropyl-5-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
Cas Number:
93904-56-4
Molecular formula:
C13H20O
IUPAC Name:
7-isopropyl-5-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
impurity 1
Chemical structure
Reference substance name:
rel-(1S,2R,4R)-1-isopropyl-4-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
Cas Number:
36208-34-1
Molecular formula:
C13H20O
IUPAC Name:
rel-(1S,2R,4R)-1-isopropyl-4-methylbicyclo[2.2.2]oct-5-ene-2-carbaldehyde
Test material form:
liquid
Specific details on test material used for the study:
Test substance name: Maceal
Chemical name: 5(or 6)-methyl-7(or 8)-(1-methylethyl)-bicyclo[2.2.2]oct-5-ene-2-carboxaldehyde
CAS number: 68259-31-4
Molecular formula: C13H20O
Molecular weight: 192 g/mol
Water solubility: 27.11 mg/L at 20 ºC
Vapour pressure: 1.647 Pa at 20 ºC 2.448 Pa at 25 ºC
Octanol water partition co-efficient (Log Pow): 3.98 to 5.41 at 25 ºC
Batch number: SC00008034
Description: Clear to Pale Yellow Liquid
Purity: 99.2%
Storage: Refrigerated (2 – 8°C), in the dark
Expiry date: 16 October 2015
Date received: 02 June 2014

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
At approximately 24-hour intervals after the start of the incubation period, pre-determined volumes of test media were removed from each incubated test vessel, and transferred to individually identified cell counting vials. The contents of each vial were diluted to a 10 mL final volume with an electrolyte solution. The cell density of the vial contents was then determined using a particle counter.

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
The test organism, Pseudokirchneriella subcapitata (Strain 278/4), was originally obtained from the Culture Collection of Algae and Protozoa (CCAP) and is a representative species of the freshwater aquatic phytoplankton. This species is recommended for testing in accordance with the OECD regulatory guidelines.
Prior to testing, duplicate starter cultures were prepared and incubated under test conditions to obtain sufficient algal cells in exponential growth and to achieve a starting algae cell density of 1 x 104 cells/mL.
Positive control tests are conducted periodically to assess the performance of the test species.

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
The laboratory temperature was set within the range 21 to 24°C and maintained within ± 2°C for the duration of the test. The temperature was recorded continuously using a digital temperature logger.
pH:
At the start of the test, the pH of freshly prepared test media, before and after alga cell inoculation was determined. The pH in each test vessel was also determined at the end of the test.
Nominal and measured concentrations:
The definitive test was conducted at nominal test concentrations of 1.0, 3.2, 10, 32 and 100% saturated solution (0.0729, 0.140, 0.311, 0.426 and 1.449 mg/L based on geometric mean measured concentrations).
Details on test conditions:
Orbital Shaker Speed: 100 rpm
Light Intensity (Lux): 6660 - 6670
Reference substance (positive control):
yes

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.912 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
other: yield
Remarks on result:
other: 95% confidence limits: 0.626-1.147 mg/L
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.877 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 95% confidence limits: 0.660 - 1.116 mg/L
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 1.449 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
0.616 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% confidence limits: 0.468-0.935
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.426 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
other: yield, biomass and growth rate
Details on results:
Based on geometric mean measured test concentrations, the 72-hour EyC50 and the 0-72 hour EbC50 and ErC50 values were calculated to be 0.912, 0.877 and greater than 1.449 mg/L, respectively. The 72-hour EyC10 and the 0-72 hour EbC10 and ErC10 values were calculated to be 0.378, 0.327 and 0.616 mg/L, respectively. The corresponding NOEC values for yield, biomass and specific growth rate after 72 hours were 0.426 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The effects of Maceal on the growth of the unicellular green alga, Pseudokirchneriella subcapitata, were determined during a 72-hour growth inhibition toxicity test conducted in accordance with OECD Chemicals Testing Guideline No. 201 Alga, Growth Inhibition Test (adopted 23 March 2006) (Annex 5 corrected 28 July 2011).
Based on geometric mean measured test concentrations, the 72-hour EyC50 and the 0-72 hour EbC50 and ErC50 values were calculated to be 0.912, 0.877 and greater than 1.449 mg/L, respectively. The 72-hour EyC10 and the 0-72 hour EbC10 and ErC10 values were calculated to be 0.378, 0.327 and 0.616 mg/L, respectively.The corresponding NOEC values for yield, biomass and specific growth rate after 72 hours were 0.426 mg/L.
The preferred observational end point in the algal growth inhibition test is growth rate because it is not dependent on the test design (ECHA guidance Chapter R.7b v1.1). The EU CLP regulation (No 1272/2008 and its adaption 286/2011) also states that classification should be based on the ErC50. The preferred observational endpoint in long-term studies is the EC10 value because it is derived from the dose response curve. In contrast the NOEC strongly depends on the experiment design (e.g. the concentrations used in the test). Thus the 72-h EC50 and EC10 based on growth rate are used for classification purposes, which were determined in this study to be > 1.499 mg/L and 0.616 mg/L respectively.