Reactive Orange 143

Partition coefficient

Link to relevant study record(s)

Reference

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12 December 2011 to 09 March 2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.8 (Partition Coefficient)

Version / remarks:

(2008)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)

Version / remarks:

(1995)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7550 (Partition Coefficient, n-octanol / H2O, Shake Flask Method)

Version / remarks:

(1996)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

shake-flask method to: flask method

Partition coefficient type:

octanol-water

Specific details on test material used for the study:

Identification FAT 40854/A TE
Description Reddish-brown powder (determined at NOTOX)
Batch TZ 5719 / BOP 02-11
Content 46.2 % (4 main constituents)
Test substance storage: At room temperature in the dark
Stability under storage conditions: Stable
Expiry date: 01 April 2016

Analytical method:

high-performance liquid chromatography

Type:

log Pow

Partition coefficient:

< -1.8

Temp.:

20 °C

pH:

7

Remarks on result:

other: Mean (n=6). Standard deviation: 4.0x10^-3. Pow: < 1.4 x 10^-2.

Details on results:

No test substance was detected in the pretreated samples from the blank mixture.  Recoveries were between 88% and 107% .

Resuls K_owof the test substance

Ratio n-octanol : water [v:v]	Analysed concentration		Kow		log Kow	pH
	n-octanol [g/l]	buffer pH 7 [g/l]	individual	mean
1:1	<0.00573	0.365	<1.6x10^-2	<1.6x10^-2	<-1.8	7.0
	<0.00579	0.351	<1.6x10^-2		<-1.8	7.0
1:2	<0.00586	0.331	<1.8x10^-2	<1.8x10^-2	<-1.8	7.0
	<0.00592	0.329	<1.8x10^-2		<-1.7	7.0
2:1	<0.00614	0.660	<9.3x10^-3	<9.3x10^-3	<-2.0	7.0
	<0.00617	0.661	<9.3x10^-3		<-2.0	7.0

No response of the test substance was detected in the n-octanol test samples.

 

 

 

 

 

Conclusions:

The log Kow of FAT 40854/A at pH 7 and 20 °C is <-1.8 (Kow <1.4 E -2).

Executive summary:

In a GLP-compliant study the partition coefficient of FAT 40854/A was determined according to EU method A.8 and OECD guideline 107, using shake flask method. In preliminary test, the content of test substance dissolved in the n-octanol sample was below the response of the lowest calibration solution. According to this the n-octanol solubility of the test substance was <5.73 mg/l. The information was sufficient for the performance of the shake flask method. Based on the main test, the Pow value of the test substance at neutral pH and 20 °C was <1.4 x 10 ^-2. It corresponds with a log Pow value of <-1.8.

Description of key information

In a GLP-compliant study the partition coefficient of FAT 40854/A was determined according to EU method A.8 and OECD guideline 107,using shake flask method. In preliminary test, the content of test substance dissolved in the n-octanol sample was below the response of the lowest calibration solution. According to this the n-octanol solubility of the test substance was <5.73 mg/l. The information was sufficient for the performance of the shake flask method. Based on the main test, the Pow value of the test substance at neutral pH and 20 °C was < 1.4 x 10^-2. It corresponds with a log Pow value of <-1.8.

Key value for chemical safety assessment

Log Kow (Log Pow):

-1.8

at the temperature of:

20 °C