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EC number: - | CAS number: -
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
Description of key information
The substance has a low potential for bioaccumulation based on the information available for its structural analogue Reaction mass of tetrasodium phosphonoethane-1,2-dicarboxylate and hexasodium-phosphonobutane-1,2,3,4-tetracarboxylate.
Key value for chemical safety assessment
- BCF (aquatic species):
- 41 dimensionless
Additional information
No data on the Bioaccumulation of the substance is available. The results from the structural analogue are used instead (for details see reporting Format as attached to section 13 in the IUCLID dossier).
One reliable key study is available on the assessment of the bioconcentration factor of the test material Reaction mass of tetrasodium phosphonoethane-1,2-dicarboxylate and hexasodium-phosphonobutane-1,2,3,4-tetracarboxylate in fish (Cyprinus carpio). The method was equivalent to that described in the OECD Guidelines for Testing of Chemicals (1996) No. 305. The test was performed according to the GLP.
The fish were exposed to 5 mg/L (named level 1) and 0.5 mg/L (named level 2) of test substance during 28 days in a flow through system. The nominal concentrations were selected after a preliminary toxicity test toOryzias latipesshowing a 96 -hours LC50 = 623 mg/L. The concentrations of test substance in test water were measured at 1, 6, 13, 20, 23 and 28 days after test initiation. The concentrations of test substance in test fish were measured at 6, 13, 20, 23 and 28 days after test initiation. As 2 peaks were detected with LC-MS analysis of the test substance, the concentration of each peak was described using the test substance concentration without consideration of the constituent ratio. The each peak was named peak 1 (comp. n=1) and peak 2 (comp. n=2) in the elution order.
No abnormality in behavior or appearance was noted during the test.
The measurements of the test substance in test water showed that the test substance was maintained at more than 94% (comp. n=1) and 83% (comp. n=2) of each nominated concentration. The variation of the concentrations of the test substance was within +/- 20% each other.
The analysis of test substance in test fish showed that at day 23 for level 1, in one replicate the concentration was found to be 24.2 µg/g for comp. n=1 and 37.6 µg/g for comp. n=2. For all other measurements, no test substance was detected and the determination limits of 15µg/g (comp. n=1) and 18 µg/g (comp. n=2) were used for the calculation of the BCF values.
In the study, as all BCFs were less than 100, it was considered that a steady-state was reached at 28th day. However, this consideration is not stated in the OECD GL, therefore the steady state assumption should be considered with caution. However, the determination limits of the analytical method are sufficiently low to conclude that the test substance has a low potential for bioaccumulation.
The BCF values calculated on the basis of the results of analysis after 20, 23 and 28 days from the initiation of exposure, are considered as follows:
Level 1 (i.e. 5 mg/L) : comp. n=1 BCF = 4.9
Level 1 (i.e. 5 mg/L) : comp. n=2 BCF = 7.6
Level 2 (i.e. 0.5 mg/L) : comp. n=1 BCF =< 28
Level 2 (i.e. 0.5 mg/L) : comp. n=2 BCF =< 41
Due to the uncertainties on the steady state and lack of details (e.g. loading rate), this study is considered as reliable with restrictions.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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