Succinic acid derivative, sodium salt

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 09, 2007 - August 01, 2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study conform with Directive 92/69/EEC, C.2, OECD Guideline No. 202

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Details on test material:

Identity: AES
Batch No.: LSF7
Purity: 80.3%
Expiration date: February 15, 2008
Solubility in water: Very soluble
Stability in water: 30 days
Aggregate state / physical
form at room temperature: Solid
Specific information: Slightly hygroscopic
Storage conditions: In a refrigerator at about 4 °C

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

For the determination of the actual test item concentrations, duplicate samples were taken from each treatment before the start of the test and at the end of the test . For the 48-hour stability samples, the contents of the respective replicates were combined prior to sampling.
All samples were stored deep-frozen (at about -20 °C) immediately after sampling.
The concentration of the test item AES was analytically measured in the duplicate test medium samples of the single test concentration taken at the start and the end of the test.
From the control, only one of the duplicate samples was analyzed from each of the sampling times.

Test solutions

Vehicle:

no

Details on test solutions:

The test medium was prepared by dissolving 30.1 mg of test item completely in 300 mL of test water intense stirring for 15 minutes at room temperature. The test medium was prepared just before introduction of the daphnids (i.e., start of the test).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Strain Straus
Source Bred in RCC Ltd ( originally supplied by University of Sheffield / UK in 1992)
Age Less than 24 hours old and were not first brood progeny
Breeding method In reconstituted water of the quality identical to the water used in the tests and under the same temperature and light conditions to those of the tests.
Kind of food Not specified in the report
Amount of food Not specified in the report
Feeding frequency Not specified in the report
Pretreatment Not specified in the report
Feeding of animals during test No feeding

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

The daphnids were observed for immobility after 24 and 48 hours of exposure.

Test conditions

Hardness:

Water Hardness: 250 as CaCO3

Test temperature:

20°C

pH:

7.8 to 8.0

Dissolved oxygen:

8.5 mg/L

Nominal and measured concentrations:

100 mg / L

Details on test conditions:

Test temperature 20 °C
Dissolved oxygen 8.5 mg/L
pH 7.9
Adjustment of pH No
Aeration of dilution water No
Quality/Intensity of irradiation Light intensity during the light period was between approximately 490 and 650 Lux.
Photoperiod 16 h of fluorescent light and 8 h of darkness cycle with a 30 minute transition period was used.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 94%

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 94%

Details on results:

No remarkable observations, clear test medium.

Results with reference substance (positive control):

In the control and at the concentration of 100 mg/L, no immobilized daphnids were determined during the test period of 48 hours

Reported statistics and error estimates:

None.

Any other information on results incl. tables

Effect of AES on the mobility of Daphnia magna

Test item concentration (mg/L)	No. of daphnids tested	Immobilized daphnids after 24 hours No.	%	Immobilized daphnids after 48 hours No.	%
Control 100	20 20	0 0	0 0	0 0	0 0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The validity criteria can be considered as fulfilled and therefore the toxicity values are considered reliable.