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EC number: 308-208-6 | CAS number: 97925-95-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 Apr - 04 Oct 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study with acceptable restrictions.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- 1) test substance concentration was not measured; 2) after 24 h temperature in 4 vessels was outside the allowed range of ± 1°C, the variation was 1.1 to 1.3 °C; 3) ASTM medium was used
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- - Stock solution: 10 mg/L of stock test medium was prepared by direct addition of Atmer 163 to ASTM medium and stirring for ca. 4 h
- Test solutions: prepared by dilution of the 10 mg/L stock solution - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: laboratory culture at Covance (culture originally obtained from Sheffield University, UK)
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: no feeding
- juveniles used were from the third brood onwards from adults
- only actively swimming individuals were used in test
CULTURING
- Medium: 500 mL ASTM standard hard water (see below: details on test conditions)
- Density: ca. 20 animals/L
- Feeding frequency: daily
- Type and amount of food: Chlorella vulgaris (ca. 5x10E5 cells/mL) plus seaweed extract as dietary supplement
- Water was renewed weekly
- Cultures were maintained up to a max. of 42 days
- New cultures were started with D. magna < 24 h
ACCLIMATION
- Acclimation period: 24 h - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 162 (0 h) - 196 mg/L (24 h) as CaCO3
- Test temperature:
- 19.5 - 20.6 °C
- pH:
- 7.7 - 8.0
- Dissolved oxygen:
- 96 - 109% air saturation
- Nominal and measured concentrations:
- nominal: control, 0.065, 0.13, 0.25, 0.5 and 1 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 150 mL glass crystallising dishes
- Renewal rate of test solution (semi-static conditions): one renewal after 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 5 animals per 100 mL test solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: culturing and testing water was a standard hard water prepared according to the standards of the American Society for Testing an Materials (ASTM) => compounds are similar to OECD guideline 202 but in lower concentration
- Composition of ASTM:
=> NaHCO3: 192 mg/L
=> CaSO4 x 2H2O: 120 mg/L
=> MgSO4 x 7H2O: 246 mg/L
=> KCl: 8 mg/L
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Photoperiod: 16 h of fluorescent lighting / 8 h dark
EFFECT PARAMETERS MEASURED:
- Effect parameter: immobilsation (swimming behaviour)
- Intervals of observations: after 24 and 48 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.85 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 0.71 - 1.02 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.59 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 0.5 - 0.7 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: 0%
- Reported statistics and error estimates:
- Probit analysis after log10 transformation of the nominal concentrations
Reference
Table 1: Immobilisation of Daphnia magna exposed to Atmer 163 over a 48 h period.
Nominal concentration [mg/L] |
Number of Daphnia magna |
Number immobilised |
|
|
|
24 h |
48 h |
Control |
20 |
0 |
0 |
0.065 |
20 |
0 |
0 |
0.13 |
20 |
0 |
0 |
0.25 |
20 |
0 |
0 |
0.50 |
20 |
1 |
6 |
1.0 |
20 |
14 |
19 |
Description of key information
EC50 (48 h): 0.59 mg/L (nominal) for Daphnia magna (OECD 202)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.59 mg/L
Additional information
The toxicity of Atmer 163 (CAS No. 97925-95-6) to aquatic invertebrates was investigated according to OECD 202 (GLP; Mattock, 2001). Daphnia magna was exposed under semi-static conditions to nominal test concentrations of 0.065, 0.13, 0.25, 0.5 and 1 mg/L. The mobility of D. magna was affected by the test substance. An EC50 of 0.59 mg/L was calculated based on mobility after an exposure period of 48 h.
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