Registration Dossier
Registration Dossier
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EC number: - | CAS number: -
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007
- Reliability:
- 1 (reliable without restriction)
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Strontium 4-chloro-2-[2-hydroxy-6-sulfonatonaphthalen-1-yl)diazenyl]benzoate
- Cas Number:
- 474814-88-5
- Molecular formula:
- C17H9ClN2O6SSr
- IUPAC Name:
- Strontium 4-chloro-2-[2-hydroxy-6-sulfonatonaphthalen-1-yl)diazenyl]benzoate
- Test material form:
- solid: nanoform, no surface treatment
Constituent 1
Sampling and analysis
- Details on sampling:
- - Concentrations: 1000 mg/l
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 18-22°C
- pH:
- 6.95
- Dissolved oxygen:
- permanent aeration
- Nominal and measured concentrations:
- nominal: 1000 mg/l
- Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Reported statistics and error estimates:
- The two control respiration rates differ not more than 15% in the study. The EC50 (3 h) of copper(II) sulphate pentahydrate is in the accepted range of 53-157 mg/l.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- the test item is not toxic at concentrations
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