3-methylsulphanilic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: as per below mentioned

Principles of method if other than guideline:

In acute toxicity to fish test been performed to evaluate the toxicological effects of chemical 4-Amino-m-toluenesulfonic acid in fish

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): 4-Amino-m-toluenesulfonic acid
- Molecular formula: C7H9NO3S
- Molecular weight: 187.218 g/mol
-Smiles: S(c1cc(c(N)cc1)C)(O)(=O)=O
- InChI: 1S/C7H9NO3S/c1-5-4-6(12(9,10)11)2-3-7(5)8/h2-4H,8H2,1H3,(H,9,10,11)
- Substance type: Organic
- Physical state: Solid

Analytical monitoring:

not specified

Details on sampling:

No data available

Vehicle:

not specified

Details on test solutions:

No data available

Test organisms (species):

Gambusia affinis

Details on test organisms:

TEST ORGANISM
- Common name:Mosquitofish
- Source:Fishes were collected from Stillwater creek in payne country
- Age at study initiation (mean and range, SD):Adult

Test type:

static

Water media type:

brackish water

Total exposure duration:

96 h

Post exposure observation period:

no data available

Hardness:

no data available

Test temperature:

19-22deg C

pH:

4.4-7.7

Dissolved oxygen:

no data available

Salinity:

no data available

Conductivity:

no data available

Nominal and measured concentrations:

no data available

Details on test conditions:

TEST SYSTEM
- Test vessel:Cylindrical pyrex jars
- Type (delete if not applicable): open / closed
- Aeration:12 inch high and 12 inchin diameter
- No. of organisms per vessel:10 fishesin 1 aquarium

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:Aquaria
- Alkalinity:100 ppm of methyl orange

OTHER TEST CONDITIONS
- Adjustment of pH:pH was lowered slightly less than 6.4


TEST CONCENTRATIONS
- Test concentrations:180,320,560,1000 mg/l

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

375 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

410 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

425 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Details on result
- Other adverse effects control: in 180 mg/l and less the fishes were apparently normal .At 320 mg/l one was dead in 48-h ,2 in 72-h and and fourth in 96-h.At 560 and 1000 mg/l all were dead in 6 hrs only.

Results with reference substance (positive control):

No data

Reported statistics and error estimates:

No data

Validity criteria fulfilled:

not specified

Conclusions:

In short term fish toxicity study the median lethal concentration (LC50) value was observed to be 375 mg/L, 410 mg/L and 425 mg/L for exposure period of 96,48 and 24-hrs respectively when tested in Gambusia affinis fish.

Executive summary:

In acute toxicity to fish test been performed to evaluate the toxicological effects of chemical 4-Amino-m-toluenesulfonic acid in fish (Gambusia affinis).The chemical was exposed to Gambusia affinis fish with dose concentration of 180,320,560,1000 mg/L with exposure duration of 24,48 and 96 hrs.The mortality was observed in the fish, at 560 and 1000 mg/L all animals were dead in 6 hrs only. Hence,the median lethal concentration (LC50) value was observed to be 375 mg/L, 410 mg/L and 425 mg/L for exposure period of  96,48 and 24-hrs respectively when tested in Gambusia affinis fish based on mortality effect.

Based on this value it can be concluded that the substance 4-amino-3-methylbenzenesulfonic acid is considered to be non-toxic to fish and can be considered to be not classified as per the CLP classification criteria.

Description of key information

In acute toxicity to fish test been performed to evaluate the toxicological effects of chemical 4-Amino-m-toluenesulfonic acid in fish (Gambusia affinis).The chemical was exposed to Gambusia affinis fish with dose concentration of 180,320,560,1000 mg/L with exposure duration of 24,48 and 96 hrs.The mortality was observed in the fish, at 560 and 1000 mg/L all animals were dead in 6 hrs only.Hence,the median lethal concentration (LC50) value was observed to be 375 mg/L, 410 mg/L and 425 mg/L for exposure period of  96,48 and 24-hrs respectively when tested in Gambusia affinis fish based on mortality effect.

Based on this value it can be concluded that the substance 4-amino-3-methylbenzenesulfonic acid is considered to be non-toxic to fish and can be considered to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

375 mg/L

Additional information

Four different studies from experimental study using peer reviewed journal and validated predicted model for short term fish toxicity endpoint of target chemical 4-amino-3-methylbenzenesulfonic acid (Cas no. 98-33-9) with relevant read across chemicals which is structurally similar to target chemical are summarized as followed:

First experimental study suggest acute toxicity to fish test been performed to evaluate the toxicological effects of chemical 4-Amino-m-toluenesulfonic acid in fish (Gambusia affinis).The chemical was exposed to Gambusia affinis fish with dose concentration of 180,320,560,1000 mg/L with exposure duration of 24,48 and 96 hrs.The mortality was observed in the fish, at 560 and 1000 mg/L all animals were dead in 6 hrs only.Hence,the median lethal concentration (LC50) value was observed to be 375 mg/L, 410 mg/L and 425 mg/L for exposure period of  96,48 and 24-hrs respectively when tested in Gambusia affinis fish based on mortality effect.

Other predicted data gives assistance to above experimental result i.e predicted model by EPI Suite ECOSAR version 1.10 indicate the 96 hours LC50 was estimated to be 230000 mg/l on Fish for substance 4-amino-3-methylbenzenesulfonic acid (Cas no. 98-33-9) on the basis of mortality effects. 

Above both results for target chemical supported by experimental study of read across 4-aminobenzenesulfonic acid (Cas no. 121-57-3) which suggest Toxic effects of test substance 4-amino-benzenesulfonic acid was determined on various fish species like Leuciscus idus, Bruchydunio rerio,Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulate for 48 and 96 hrs in static condition and in fresh water. During experiment the lethal concentrations i.e LC0 and LC50 values were found to be 1000 mg/l and 100.4 mg/l respectively. (Chemosphere, Vol. 28, No. 12, pp. 2203.2236, 1994)

Another study for one more structurally similar read across chemical 2-amino-5-methylbenzenesulfonic acid (Cas no. 88-44-8) indicate the toxic effects of test substance 4-aminotoluene-3-sulphonic acid was examined on fish Oryzias latipes for 48 hrs. During experiment the lethal concentration (LC50) was found to be 480 mg/l on the basis of mortality effects.

All available median lethal concentration (LC50) values for fish toxicity in acute exposure is in the range of 375-230000 mg/l which gives common conclusion as target chemical 4-amino-3-methylbenzenesulfonic acid (Cas no. 98-33-9) is considered to be non-toxic to fish and can be considered to be not classified for aquatic environment as per the CLP classification criteria.