Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of aluminium, 2-(1,3-dihydro-3-oxo-5-sulfo-2H-indol-2-ylidene)-2,3-dihydro-3-oxo-1H-indole-5-sulfonic acid complex on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (as cited in study report): C.I. Food Blue 1- Molecular formula (if other than submission substance): C16H8N2S2O8Na2- Molecular weight (if other than submission substance): 449.375 g /mol- Substance type: Organic- Physical state: Dark-blue powder or granules- Smiles notation (if other than submission substance): C1(=C2\C(c3cc(S(O)(=O)=O)ccc3N2)=O)\C(c2cc(S(O)(=O)=O)ccc2N1)=O.[Al+3]
Analytical monitoring:
not required
Remarks:
substance is stable
Vehicle:
not specified
Details on test solutions:
The test substance was soluble in water; In house solubility was found to be 339.2 mg/L. Therefore the stock solution prepared as 400mg/4L, with the concentration of 100mg/L, and was kept for 48 hr. stirring.After this filter the stock, gave it for analytical detection and then stock taken for experiment.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM- Common name:Zebra Fish - Strain:Danio rerio- Source: Unique Aqua Fish Corner- Length at study initiation (length definition, mean, range and SD): average 1.9 cm- Weight at study initiation (mean and range, SD): average 0.0699 g g -Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.-Duration of housing: 15 days -Photoperiod: 16 hours light and 8 hours dark-Diet : Standard brand feed, once daily-Acclimatization: seven days -feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
158.4 mg of CaCO3
Test temperature:
23 °C
pH:
7.43
Nominal and measured concentrations:
100 mg/l
Details on test conditions:
TEST SYSTEM - Test vessel: Plastic aquaria - Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water - Aeration: Aeration in test vessels was provided 1 day before the start of experiment - No. of organisms per vessel: 8 fishes OTHER TEST CONDITIONS - Photoperiod: 16 hours light and 8 hours dark
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other:
Sublethal observations / clinical signs:

8.1. Mortality and symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

-

-

-

-

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

pH and oxygen content:

Nominal

Concentration

(mg/l)

Number of

fish

pH readings after

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

7.20

7.22

8.33

8.30

8.87

8.38

8.35

8.39

100

4

4

7.45

7.48

7.71

7.76

7.95

7.76

7.91

7.59

Nominal

Concentration

(mg/l)

Number of

fish

Temperature (°C) after

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

21

21

25

25

24

24

23

23

100

4

4

21

21

25

25

24

24

23

23

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.16521-38-3 on Zebra Fish Danio rerio was determine to be >100 mg/l
Executive summary:

Study was conducted to access the effect of test chemical aluminium, 2-(1,3-dihydro-3-oxo-5-sulfo-2H-indol-2-ylidene) -2,3-dihydro-3-oxo-1H-indole-5-sulfonic acid complex on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

The test substance was soluble in water; In house solubility was found to be 339.2 mg/L. Therefore the stock solution prepared as 400mg/4L, with the concentration of 100mg/L, and was kept for 48 hr. stirring.After this filter the stock, gave it for analytical detection and then stock taken for experiment. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item aluminium, 2-(1,3-dihydro-3-oxo-5-sulfo-2H-indol-2-ylidene)-2,3-dihydro-3-oxo-1H-indole-5-sulfonic acid complex. to various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemicalaluminium, 2-(1,3-dihydro-3-oxo-5-sulfo-2H-indol-2-ylidene) -2,3-dihydro-3-oxo-1H-indole-5-sulfonic acid complexon the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

The test substance was soluble in water; In house solubility was found to be 339.2 mg/L. Therefore the stock solution prepared as 400mg/4L, with the concentration of 100mg/L, and was kept for 48 hr. stirring.After this filter the stock, gave it for analytical detection and then stock taken for experiment.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test itemaluminium, 2-(1,3-dihydro-3-oxo-5-sulfo-2H-indol-2-ylidene)-2,3-dihydro-3-oxo-1H-indole-5-sulfonic acid complex.to various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information

Short term toxicity to fish:

Study was conducted to access the effect of test chemicalaluminium, 2-(1,3-dihydro-3-oxo-5-sulfo-2H-indol-2-ylidene) -2,3-dihydro-3-oxo-1H-indole-5-sulfonic acid complexon the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

The test substance was soluble in water; In house solubility was found to be 339.2 mg/L. Therefore the stock solution prepared as 400mg/4L, with the concentration of 100mg/L, and was kept for 48 hr. stirring.After this filter the stock, gave it for analytical detection and then stock taken for experiment.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test itemaluminium, 2-(1,3-dihydro-3-oxo-5-sulfo-2H-indol-2-ylidene)-2,3-dihydro-3-oxo-1H-indole-5-sulfonic acid complex.to various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.