[8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2-naphthyl]trimethylammonium chloride

Density

Link to relevant study record(s)

Reference

Endpoint:

density

Remarks:

density

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental result performed using standard methods of analysis

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Principles of method if other than guideline:

The density is calculated from the difference in weight between the full and empty pycnometer and its volume

GLP compliance:

no

Type of method:

pycnometer method

Specific details on test material used for the study:

- Name of test material:(8-((4-Amino-3-nitrophenyl)azo)-7-hydroxy-2-naphthyl)trimethylamm- onium chloride
- Molecular formula: C19H20N5O3Cl
- Molecular weight: 401.852 g/mol
- Smiles notation: [N+](c1cc(\N=N\c2c3cc([N+](C)(C)C)ccc3ccc2O)ccc1N)([O-])=O.[ClH-]
- Substance type: Organic
- Physical state: Solid

Key result

Type:

pour density

Density:

0.557 g/cm³

Temp.:

28.4 °C

Key result

Type:

tap density

Density:

0.716 g/cm³

Temp.:

28.4 °C

Method Details		Observations
Guideline	OECD 109	1^stReading	2^ndReading
Method	Test methods: Pour density and tapped density of powders” recommended in The International Pharmacopoeia, Fourth Edition, 2014
Principle of method	Pour Density:It is the ratio of known weight of test item and its volume in graduated glass cylinder. Tap Density: It is the difference between the volume of test item measured before & after tapping in the graduated glass cylinder.
Test temperature (°C)		27.4 °C	29.4 °C
Atmospheric pressure (in hPa)		973.4	972.5
Weight of test item(m)		10.027	10.027
Volume of measuring cylinder; readability in g		250ml ; 2 ml	250 ml; 2ml
Volume of test item without tapping (V)		18	18
Volume of test item after tapping ( V1)	After 10 tapping	14	14
	After 50 tapping
	After 100 tapping
	After 250 tapping	-	-
	After 500 tapping	-	-
	After 750 tapping	-	-

 

CALCULATIONS:

By Pour Density and Tapped Density Method:

Parameters	Details	Calculated values		Average Density
		1st Calculation	2nd Calculation
Pour Density of test Item (g/cm³)	m/v	0.5570	0.5570	0.5570
Tap density of test Item (g/cm³)	m/v	0.7160	0.7162	0.7162

Conclusions:

The density of test item [8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2- naphthyl]trimethylammonium chloride (CAS No. 68391-32-2)was determined following OECD Guideline 109 and according to the Test methods: Pour density and tapped density of powders” recommended in The International Pharmacopoeia, Fourth Edition, 2014 using bulk density apparatus . The mean pour density and tapped density of test item was determined to be 0.5570 g/cm³ and 0.7162 g/cm³at 28.4°C and 972.9 hPa.

Executive summary:

The density of test item [8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2- naphthyl]trimethylammonium chloride (CAS No. 68391-32-2)was determined following OECD Guideline 109 and according to the Test methods: Pour density and tapped density of powders” recommended in The International Pharmacopoeia, Fourth Edition, 2014 using bulk density apparatus . The mean pour density and tapped density of test item  was determined to be 0.5570 g/cm³ and 0.7162 g/cm³at 28.4°C and 972.9 hPa.

Description of key information

The density of test item [8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2- naphthyl]trimethylammonium chloride (CAS No. 68391-32-2)was determined following OECD Guideline 109 and according to the Test methods: Pour density and tapped density of powders” recommended in The International Pharmacopoeia, Fourth Edition, 2014 using bulk density apparatus . The mean pour density and tapped density of test item  was determined to be 0.5570 g/cm³ and 0.7162 g/cm³at 28.4°C and 972.9 hPa.

Key value for chemical safety assessment

Relative density at 20C:

0.557

Additional information

The density of test item [8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2- naphthyl]trimethylammonium chloride (CAS No. 68391-32-2)was determined following OECD Guideline 109 and according to the Test methods: Pour density and tapped density of powders” recommended in The International Pharmacopoeia, Fourth Edition, 2014 using bulk density apparatus . The mean pour density and tapped density of test item  was determined to be 0.5570 g/cm³ and 0.7162 g/cm³at 28.4°C and 972.9 hPa.

Other supporting study from review article also indicates that the density of chemical (8-((4-Amino-3-nitrophenyl)azo)-7-hydroxy-2-naphthyl)trimethylamm- onium chloride was determined to be 1.7 g/cm3.

As the data from lab has K1 reliability and the test was performed using OECD guidelines which has the value of density as 0.5570 g/cm3 (pour density) and 0.7162 g/cm3 (tapped density) to,thus considered as key study for the chemical safety assessment