Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl)azo]-4-hydroxynaphthalene-1-sulphonate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer review journal

Data source

Reference

Reference Type:

publication

Title:

Biochemical decomposition of coal tar dyes II. Acute toxicity of coal tar dyes and their decomposed products

Author:

Yasuhide TONOGAI, Masahiro IWAIDA, Masatomo TATI, Youki OSE and Takahiko SATO

Year:

1978

Bibliographic source:

The Journal of Toxicological Science, Vol . 3 205-214, 1978

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: refer below principle

Principles of method if other than guideline:

TLm test were performed on Himedaka (Oryzias latipes) for measuring survival test.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl)azo]-4-hydroxynaphthalene-1-sulphonate

EC Number:

224-909-9

EC Name:

Disodium 3-[(2,4-dimethyl-5-sulphonatophenyl)azo]-4-hydroxynaphthalene-1-sulphonate

Cas Number:

4548-53-2

Molecular formula:

C18H16N2O7S2.2Na

IUPAC Name:

disodium 3-[(2,4-dimethyl-5-sulfonatophenyl)diazenyl]-4-hydroxynaphthalene-1-sulfonate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): Ponceau SX- Molecular formula (if other than submission substance): C18H16N2O7S2.2Na- Molecular weight (if other than submission substance): 480.4276- Smiles notation (if other than submission substance): [Na+].[Na+].Cc1cc(C)c(cc1N=Nc2cc(c3ccccc3c2O)S(=O)(=O)[O-])S(=O)(=O)[O-]- InChl (if other than submission substance): 1S/C18H16N2O7S2.2Na/c1-10-7-11(2)16(28(22,23)24)8-14(10)19-20-15-9-17(29(25,26)27)12-5-3-4-6-13(12)18(15)21;;/h3-9,21H,1-2H3,(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2/b20-19+;;- Substance type: Organic- Physical state: Solid

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

No data

Test solutions

Vehicle:

not specified

Details on test solutions:

No data

Test organisms

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM- Common name: Himedaka- Strain: No data available- Source: No data available- Age at study initiation (mean and range, SD): No data available- Length at study initiation (length definition, mean, range and SD): About 2 cm long, - Weight at study initiation (mean and range, SD): weight ca. 0.2 g- Method of breeding: No data available- Feeding during test No data available- Food type: No data available- Amount: No data available- Frequency: No data availableACCLIMATION- Acclimation period: 10 days- Acclimation conditions (same as test or not): No data available- Type and amount of food: No data available- Feeding frequency: No data available- Health during acclimation (any mortality observed): No data availableQUARANTINE (wild caught)- Duration: No data available- Health/mortality: No data available

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Post exposure observation period:

No data

Test conditions

Hardness:

No data

Test temperature:

20 deg.C

pH:

7

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

No data

Details on test conditions:

TEST SYSTEM- Test vessel: Fish tank- Type (delete if not applicable): closed- Material, size, headspace, fill volume:- Aeration: Aeration with bubbler- Type of flow-through (e.g. peristaltic or proportional diluter):No data available- Renewal rate of test solution (frequency/flow rate): No data available- No. of organisms per vessel: 10 fishes- No. of vessels per concentration (replicates): No data available- No. of vessels per control (replicates): No data available- No. of vessels per vehicle control (replicates): No data available- Biomass loading rate: No data availableTEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: No data available- Total organic carbon: No data available- Particulate matter: No data available- Metals: No data available- Pesticides: No data available- Chlorine: No data available- Alkalinity: No data available- Ca/mg ratio: No data available- Conductivity: No data available- Culture medium different from test medium: No data available- Intervals of water quality measurement: No data availableOTHER TEST CONDITIONS- Adjustment of pH: No data available- Photoperiod: No data available- Light intensity: No data availableEFFECT PARAMETERS MEASURED (with observation intervals if applicable) :BOD,CODTEST CONCENTRATIONS- Spacing factor for test concentrations: No data available- Justification for using less concentrations than requested by guideline: No data available- Range finding study No data available- Test concentrations: 3000mg/kg- Results used to determine the conditions for the definitive study No data available

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

3 000 other: mg/kg

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: Tolerance effect

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The TLm (LC 50) value for the short term toxicity to fish was found to be greater than 3000mg/kg.

Executive summary:

Short term toxicity test was performed on Himegaka(Oryzias latipes)was used and acclimated for 10 days in tap water before experiment. In lab, 1 L solution of pH 7 containing 3,000 mg/litre of a Ponceau SX to be tested. 10 tested fish were kept in the tank without direct sunlight for 48 hrs and survival rate was determined.

 

From the experiment, TLm (LC 50) value for the short term toxicity to fish was found to be greater than 3000mg/kg.