2-Acetone, polymer with phenol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to O.E.C.D. Testing Guideline No. 203 with GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-Acetone, polymer with phenol

EC Number:

500-086-4

EC Name:

2-Acetone, polymer with phenol

Cas Number:

35238-34-7

Molecular formula:

(C6H6O.C3H6O)x

IUPAC Name:

2-Propanone, polymer with phenol

Constituent 2

Reference substance name:

2-Acetone polymer with phenol

IUPAC Name:

2-Acetone polymer with phenol

Constituent 3

Reference substance name:

: 2-Propanone, polymer with phenol

IUPAC Name:

: 2-Propanone, polymer with phenol

Constituent 4

Reference substance name:

BPA-Tars

IUPAC Name:

BPA-Tars

Details on test material:

As per IUCLID5 Sections 1.1. -1.4.

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Test solutions were sampled for BPA-Tars concentration determination at 0 and 96 hr.

Test solutions

Vehicle:

yes

Details on test solutions:

Required amounts of BPA-Tars were added to 5 litres of dilution water and treated for one hour in an ultrasonic bath and afterwards stirred for 24 h on magnetic stirrers. The nominal concentrations were: 0, 6.25, 12.5, 25, 50 and 100 mg/L.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Zebra fish (Danio rerio) were acquyired from Interaquaristik (Germany). The fish were maintained at 20-24 °C with a dissolved oxygen content of > 5 mg/L and feed commercial fish food, daily. upto 24 h prior to test initiation.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Test conditions

Hardness:

14.8°dH (= 264 mg/L CaCO3).

Test temperature:

21.9 - 22.7 degrees C.

pH:

7.5 - 7.9

Dissolved oxygen:

The dissolved oxygen concentration remained above 60% of the air-saturation value throughout the exposure period.

Salinity:

no data, freshwater

Nominal and measured concentrations:

Nominal concentrations were: 6.25, 12.5, 25, 50 and 100 mg/L and the measured concentrations ranged from 25.9% - 28.1% of the nominal concentration.

Details on test conditions:

Fish were exposed in glass aquaria holding 5 L of test media covered with glass plates with 7 animals per test concentration. there was no feeding during the exposure period of 96 hr. Exposure was by a static system with gentle aeration via narrow glass tubes.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4.95 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: the LC50 was 4.95 mg/L from 24 hr to 96 hr.

Details on results:

The initial LC50 value at two hr exposure was 19.8 mg/L. The final LC50 value was 4.95 mg/L after 24 hr of exposure

Results with reference substance (positive control):

No data

Reported statistics and error estimates:

The LC 50 was calculated by probit analysis using statistic programm ToxRat Professional version 2.10.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

BPA-Tars was demonstrated to have an acute LC50 for the Zebrafish of 4.95 mg/L from 24 to 96 hr of exposure. This finding suggest that BPA-Tars requires Classification and Labeling as Toxic to aquatic organisms.

Executive summary:

2 -Acetone polymer with phenol (BPA-Tars) was assessed for acute toxicity to the Zebrafish by an O.E.C.D. Testing Guideline No. 203 study with GLP comlpliance. BPA-Tars was demonstrated to have an acute LC50 for the Zebrafish of 4.95 mg/L from 24 to 96 hr of exposure. This finding suggest that BPA-Tars requires Classification and Labeling as Toxic to aquatic organisms.