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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Only basic information given.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1960

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
In an acute dermal toxicity study, the shaved skin of 6 male mice were exposed to the test item with 0.25 % esterine over a period of 2 hours. The detection of the esterine resorption was used as a indirect proof of the inclusion of the solvent.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
mouse
Strain:
not specified
Sex:
male
Details on test animals and environmental conditions:
no data

Administration / exposure

Type of coverage:
occlusive
Details on dermal exposure:
TEST SITE
- Area of exposure: The contact area was limited by small cylindrical glass containers (diameter 17, height 10 mm) which were closed at the top with waterproof adhesive tape and fixed with commercially available glue on the abdominal skin. Thus, the area of exposure was 2.2 cm².
Duration of exposure:
2 hours
Doses:
Test substance was tested as solution agent together with 0.25 % eserine.
No. of animals per sex per dose:
6
Control animals:
no
Details on study design:
No data
Statistics:
No data

Results and discussion

Mortality:
No mortality occurred.
Clinical signs:
No data
Body weight:
No data
Gross pathology:
No data

Any other information on results incl. tables

Results of the resorption test of the test item with 0.25 % eserine

Solution agent for eserine (0.25 % solution)

Minutes to increase the lifting height (mean)

More minutes until reaching the maximum lift height (mean)

Number of analyzed trials

Test item

- (10)

-

6

- An application of the test item alone also did not lead to an intoxication.

Applicant's summary and conclusion

Interpretation of results:
study cannot be used for classification
Conclusions:
In the present study, the test item showed no dermal absorption after an exposure period of 2 hours in mice. Thus, a toxic effect at this dose can be excluded under the experimental conditions chosen.
Executive summary:

In this study the percutane resorption of the test item with 0.25 % eserine was observed. The shaved skin of 6 male mice were exposed to the test item with 0.25 % eserine over a period of 2 hours. The detection of the eserine resorption was used as an indirect proof of the inclusion of the solvent. Eserine was not absorbed under the said experimental conditions. It can be concluded that the test item was also not absorbed. Thus, a toxic effect of the test item at this dose can be excluded under the experimental conditions chosen. An exposure to the test item alone also did not lead to an intoxication.