Benzyl formate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Only basic information given.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1960

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

In an acute dermal toxicity study, the shaved skin of 6 male mice were exposed to the test item with 0.25 % esterine over a period of 2 hours. The detection of the esterine resorption was used as a indirect proof of the inclusion of the solvent.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Benzyl formate

EC Number:

203-214-4

EC Name:

Benzyl formate

Cas Number:

104-57-4

Molecular formula:

C8H8O2

IUPAC Name:

benzyl formate

Test animals

Species:

mouse

Strain:

not specified

Sex:

male

Details on test animals or test system and environmental conditions:

no data

Administration / exposure

Type of coverage:

occlusive

Details on dermal exposure:

TEST SITE
- Area of exposure: The contact area was limited by small cylindrical glass containers (diameter 17, height 10 mm) which were closed at the top with waterproof adhesive tape and fixed with commercially available glue on the abdominal skin. Thus, the area of exposure was 2.2 cm².

Duration of exposure:

2 hours

Doses:

Test substance was tested as solution agent together with 0.25 % eserine.

No. of animals per sex per dose:

6

Control animals:

no

Details on study design:

No data

Statistics:

No data

Results and discussion

Mortality:

No mortality occurred.

Clinical signs:

No data

Body weight:

No data

Gross pathology:

No data

Any other information on results incl. tables

Results of the resorption test of the test item with 0.25 % eserine

Solution agent for eserine (0.25 % solution)	Minutes to increase the lifting height (mean)	More minutes until reaching the maximum lift height (mean)	Number of analyzed trials
Test item	- (10)	-	6

- An application of the test item alone also did not lead to an intoxication.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

In the present study, the test item showed no dermal absorption after an exposure period of 2 hours in mice. Thus, a toxic effect at this dose can be excluded under the experimental conditions chosen.

Executive summary:

In this study the percutane resorption of the test item with 0.25 % eserine was observed. The shaved skin of 6 male mice were exposed to the test item with 0.25 % eserine over a period of 2 hours. The detection of the eserine resorption was used as an indirect proof of the inclusion of the solvent. Eserine was not absorbed under the said experimental conditions. It can be concluded that the test item was also not absorbed. Thus, a toxic effect of the test item at this dose can be excluded under the experimental conditions chosen. An exposure to the test item alone also did not lead to an intoxication.