(±)-13-ethyl-3-methoxygona-1,3,5(10),8-tetraen-17-one

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Jul 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

Not relevant

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(±)-13-ethyl-3-methoxygona-1,3,5(10),8-tetraen-17-one

EC Number:

227-699-7

EC Name:

(±)-13-ethyl-3-methoxygona-1,3,5(10),8-tetraen-17-one

Cas Number:

5941-92-4

Molecular formula:

C20H24O2

IUPAC Name:

13-ethyl-3-methoxy-6,7,11,12,13,14,15,16-octahydro-17H-cyclopenta[a]phenanthren-17-one (non-preferred name)

Details on test material:

- Name of test material (as cited in study report): Ethyltetraenon
- Analytical purity: 95.9%
- Lot/batch No.: 11614001

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Two immobilized Daphnia (Le. 10%) were observed in the control, whereas no immobilization was observed in the graup exposed to the test compound.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The immobilization of 10% in the contral is acceptable according to the OECD guideline.
The EC50/48 hours of ZK 47568 to Daphnia magna is'higher than a saturated solution (1 mg/L).

Executive summary:

The purpose of this study was to determine the acute toxicity (immobilization, EC50/48 hours) of ZK 47568 to Daphnia magna in a limit-test. The study was conducted in agreement with the test guideline of the OECD, no. 202, "Daphnia spec., acute immobilization test".

Twenty Daphnia were used for the test solution of Ethyltetraenon and twenty for the control group. The Daphnia were exposed to the test substance and the dilution water (tap water) for aperiod of 48 hours under static conditions in a limit-test. Imrmobilization was recorded at 24 hours and 48 hours. For the preparation of the test solution 100 mg/L of the test substance were suspended and stirred for 24 hours. Then it was filtered through a glassfibrefilter (poresize 0.45 um). The saturated solution obtained by this procedure was used for the test. Sam pies for a TOC analysis were taken at 0 and 48 hours. The TOC analysis gave a concentration of Ethyltetraenon of 1 mg/L based on a calculation using the molecular formula.

Two immobilized Daphnia (i.e. 10%) were observed in the control, whereas no immobilization was observed in the group exposed to the test compound.