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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Jul 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: According to guideline under GLP
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1996

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Principles of method if other than guideline:
Not relevant
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Ethyltetraenon
- Analytical purity: 95.9%
- Lot/batch No.: 11614001

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 1 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Two immobilized Daphnia (Le. 10%) were observed in the control, whereas no immobilization was observed in the graup exposed to the test compound.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The immobilization of 10% in the contral is acceptable according to the OECD guideline.
The EC50/48 hours of ZK 47568 to Daphnia magna is'higher than a saturated solution (1 mg/L).
Executive summary:

The purpose of this study was to determine the acute toxicity (immobilization, EC50/48 hours) of ZK 47568 to Daphnia magna in a limit-test. The study was conducted in agreement with the test guideline of the OECD, no. 202, "Daphnia spec., acute immobilization test".

Twenty Daphnia were used for the test solution of Ethyltetraenon and twenty for the control group. The Daphnia were exposed to the test substance and the dilution water (tap water) for aperiod of 48 hours under static conditions in a limit-test. Imrmobilization was recorded at 24 hours and 48 hours. For the preparation of the test solution 100 mg/L of the test substance were suspended and stirred for 24 hours. Then it was filtered through a glassfibrefilter (poresize 0.45 um). The saturated solution obtained by this procedure was used for the test. Sam pies for a TOC analysis were taken at 0 and 48 hours. The TOC analysis gave a concentration of Ethyltetraenon of 1 mg/L based on a calculation using the molecular formula.

Two immobilized Daphnia (i.e. 10%) were observed in the control, whereas no immobilization was observed in the group exposed to the test compound.