Reaction mass of 3-[(3aS,7aR)-octahydro-1H-4,7-methanoinden-1-yl]propanal and 3-[(2s,3aR,7aS)-octahydro-1H-4,7-methanoinden-2-yl]propanal and 3-[(2r,3aR,7aS)-octahydro-1H-4,7-methanoinden-2-yl]propanal

Short-term toxicity to aquatic invertebrates

Description of key information

A study was performed to assess the acute toxicity of the substance to Daphnia magna. The study was conducted in accordance with OECD Guideline for Testing of Chemicals No. 202.
Based on the results of a preliminary range-finding test, Water Accommodated Fractions (WAFs) were prepared with individual loading rates of 0.22, 0.46, 1.0, 2.2, 4.6 and 10 mg/L
For each concentration and a control group, twenty Daphnia magna (less than 24 hours old) were exposed for 48 hours. The exposure took place in airtight closed vessels with a headspace reduced to a minimum and in the dark. The incidence of immobilisation was recorded for each test and control group at 24 hours and at 48 hours.
Samples taken from all concentrations were analysed with a validated GC-FID method. The actual concentrations measured at the start of the test increased with the applied loading rate. The measured concentrations decreased to 24-89% of the initial at the end of the test. Stronger decrease was observed at the lower concentrations. Based on these results, the following average exposure concentrations were calculated: 0.082, 0.29, 0.83, 1.5, 3.3 and 9.0 mg/L.
In order to explain the decrease of concentrations in this test, the reserve samples taken at the start and at the end of the exposure from WAF prepared at 10 mg/L were analysed by UPLC-UV for presence of oxidation products. No oxidation product of test substance was detected in the samples. Hence, it can be concluded that the decrease of the concentration was caused by other processes than oxidation.
The measured 48h-EC50 value with Daphnia magna was 0.81 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.81 mg/L