Polyhaloalkene

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Sampling method: t=0, t=48hr
- Sample storage conditions before analysis: 2-10C and shpped at end of study

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
In a sparging system, the test material was purged through test medium for different periods at a rate of approximately 20 mL er minute. The gas flow rate was assesseed using a soap- bubble flow meter. Air was simultaneoulsy purged to maintain acceptable levels of dissolved oxygen.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: in house laboratory culture with known history
young daphnia less than 24 hours old from parental daphnids older than 2 weeks of age

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

pH:

start of test pH 7.9 - 8.0
48 hrs - pH 7.8 - 8.0

Dissolved oxygen:

start of test 5.7 - 9.6
48 hr 6.0 -9.0

Nominal and measured concentrations:

nominal concentration - number of minutes of purging test material at 20 mL/min
measured - below limit except at 60 minutes purging resulting in a measured concentration of 83 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type :closed
- Material, size, headspace, fill volume: 100 ml, all glass, filled up to rim
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per vehicle control (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod: 16 hours

Reference substance (positive control):

yes

Remarks:

K2Cr2O7

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

83 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: none

- Other biological observations: none
- Mortality of control:none
- Other adverse effects control:none
- Abnormal responses: none

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Mortality: yes
- EC50/LC50: 48 hr - 0.51 mg/L

Validity criteria fulfilled:

yes

Remarks:

no daphnia immobilized in control, oxygen concentration above 5 mg/L at end of test, temperature and pH maintained, potassium dichromate results within acceptable range

Conclusions:

Under the conditions of the present test, the 48 hr EC50 for daphnia magna exposed to the test substance was > 83 mg/L. Since no effect was recorded at this level, it can be safely concluded that the 48 hr EC50 for the test substance is > 100 mg/L and the test substance can be classified as not harmful for crustaceans.

Description of key information

HFO-1234yf has low toxicity to daphnia

Additional information

HFO-1234yf has low toxicity to daphnia magna with an EC50 greater than 100 mg/l.