Polyhaloalkene

Long-term toxicity to fish

Administrative data

Endpoint:

fish, juvenile growth test

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

The dissolved oxygen concentrations were not maintained at 70% or more of the air saturation value throughout the test, this was a consequence of testing a gas in air-tight closed vessels. However, no negative effects were observed.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 215 (Fish, Juvenile Growth Test)

Version / remarks:

2000

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.14 (Fish Juvenile Growth Test)

Version / remarks:

2008

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

468-710-7

EC Name:

-

Cas Number:

754-12-1

Molecular formula:

C3H2F4

IUPAC Name:

2,3,3,3-tetrafluoroprop-1-ene

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: All test concentrations and stock solution
- Sampling method: The samples were taken in a 20 ml GC-vial that already contained 0.5 ml acetone.
- Sample storage conditions before analysis: none

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of stock solutions by loading two 10 L gas sampling bags each with 7 or 7.5 litres of ISO medium followed by addition of ~3.5 or 3.75 grams gas (500 mg/l). The gas sampling bags were closed immediately after addition and then shaken carefully for 4 hours on a platform shaker. Subsequently, the bags were left to settle overnight. The two saturated media (expected concentration ~160 mg/l) were mixed and then used for preparing the test concentrations by serial dilution.

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands.
- Weight at study initiation (mean): 0.76 ± 0.08 g

FEEDING DURING TEST
- Food type: Cyprico Crumble Excellent (300-500 um), Coppens International bv, Helmond, The Netherlands).
- Amount: 4% of body weight per vessel
- Frequency: daily

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

28 d

Test conditions

Hardness:

211.5 mg/L

Test temperature:

20.0 - 21.6 °C

pH:

6.9 - 7.9

Dissolved oxygen:

In freshly prepared solutions: 8.3 - 10.1 mg/L
In 24 h old solutions: 2.1 - 6.2 mg/L
In 24 h old control: 1.8 - 6.2 mg/L

Nominal and measured concentrations:

Nominal test substance concentrations: 1.5, 3.0, 6.0, 12 and 24 mg/L
Mean measured substance concentrations: 1.2, 2.7, 5.4, 11 and 22 mg/L (82 - 91% of nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: airthight glass vessels (40 L)
- Type: closed
- Fill volume: 38 L
- Aeration: none
- Renewal rate of test solution (frequency): daily
- No. of organisms per vessel: 16
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.32 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Adjusted ISO medium, formulated using RO-water (tapwater purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands)

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Daily determination of mortalities, clinical sings were determined three times a week, body weight was determined at test start and after 14 and 28 days of exposure respectively.

Reference substance (positive control):

yes

Remarks:

penta-chlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

28 d

Dose descriptor:

EC50

Effect conc.:

> 22 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

2.7 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

28 d

Dose descriptor:

LOEC

Effect conc.:

5.4 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

- Type of and number with morphological abnormalities: none
- Type of and number with behavioural abnormalities: none
- Other biological observations: no mortalities observed
Statistical analysis on the growth rates for the interval of days 0-14 showed that the growth rate of the fish exposed to test groups of 5.4 and 22 mg/l were statistically significant lower than the growth of the fish in the control group.

Results with reference substance (positive control):

The reference test showed normal sensitivity of the supplied carp.

Reported statistics and error estimates:

ANOVA, Tukey test, Bonferroni t-test, TOXSTAT Release 3.5, 1996, D.D. Gulley, A.M. Boelter, H.L. Bergman

Any other information on results incl. tables

Table 1: Mean fish weights recorded at the start, after 14 and 28 days.

Day of recording	Mean fish weights (g):
	Control	1.2 mg/L	2.7 mg/L	5.4 mg/L	11 mg/L	22 mg/L
0	0.76	0.79	0.78	0.77	0.78	0.73
14	1.11	1.11	1.10	1.06	1.10	1.01
28	1.49	1.48	1.49	1.40	1.45	1.27
% increase on day 28*	196	189	191	182	186	174

*Based on non-rounded figures.

Table 2: Mean growth rates recorded for the intervals of 0-14 days and 0-28 days.

Interval	Mean growth rates:
	Control	1.2 mg/L	2.7 mg/L	5.4 mg/L	11 mg/L	22 mg/L
Day 0-14	2.71	2.45	2.48	2.22	2.47	2.32
Day 0-28	2.42	2.28	2.32	2.11	2.20	1.96