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EC number: 251-649-3
CAS number: 33704-61-9
- Clinical observations and mortality data: There were no deaths. No
signs of systemic toxicity were noted in the test or control animals
during the test.
- Bodyweight: Bodyweight changes of the test animals between Day 1 and
Day 6 were comparable to those observed in the corresponding control
group animals over the same period.
- An EC3 value of 33% was calculated using this equation: EC3 = c +
[[(3-d)/(b-d)] x (a-c)].
This Local Lymph Node Assay (OECD 429) was performed to determine
the sensitising potential of Cashmeran in mice. Groups of 5 mice were
treated with the undiluted Cashmeran or Cashmeran at concentrations of
50% or 25% v/v in acetone/olive oil 4:1.α-Hexylcinnamaldehyde,
freshly prepared as a 25% v/v dilution in acetone/olive oil 4:1, was
used as positive control. Clinical observations and bodyweights were
recorded and lymph node proliferation was determined using 3HTdR
The number of radioactive disintegrations per minute (dpm) reflect
the proliferation reponse of lymph node cells, and were 2295.90,
6168.85, 8413.63, and 8073.27 mean dpm/animal for the 0%, 25%, 50%, and
100% concentration groups, respectively. This corresponds with a lymph
node proliferation of 2.69, 3.66 and 3.52, respectively, for the
Cashmeran-treated groups, calculated as the Stimulation Index (SI). No
mortality and no signs of systemic toxicity were observed. No effects on
bodyweight (gain) were observed.
An EC3 value (the concentration of test item expected to cause a 3
-fold increase in 3HTdR incorporation) of 33% was calculated. Based on
the absence of effects at the lowest concentration tested, a NOEC of 25%
could be established.
Under the conditions of this study, a maximum SI of 3.66 was
calculated. Considering the SI threshold of 3 as stated in Annex I of
1272/2008/EC and Annex VI of 67/548/EEC, the substance needs to be
classified as sensitiser for concentrations ≥ 33%.
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