N-(4-amino-9,10-dihydro-3-methoxy-9,10-dioxo-1-anthryl)benzenesulphonamide

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: EEC Method No. L 133/118

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 9300102
- Expiration date of the lot/batch: 07/98

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

Stock solution
The concentration of the stock "solution" is 0.5 g/l. It was prepared using a 10 minutes ultrasonic treatment for homogenisation.

Test organisms (species):

activated sludge

Details on inoculum:

- Organism : Activated sludge of a communal sewage treatment plant (ARA-Birsig Therwil)
- Pretreatment:3 times washing with tap water and addition of synthetic sewage (a day before the main test)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Post exposure observation period:

Not available

Test temperature:

21.0 °C

pH:

7.8 before the test beginning

Nominal and measured concentrations:

320, 100, 32 and 10 mg/l of the test substance
32, 10 and 3.2 mg/l of the reference substance

Details on test conditions:

- Aeration: 0.1 - 0.2 l/min by Pasteur-pipette

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol (Merck 821768)

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

IC50 (3 h) of the reference substance 3,5-Dichlorophenol : 13.0 mg/l

Validity criteria fulfilled:

yes

Conclusions:

IC50 (3 h) of the FAT 36034/D FAT 36034/D is determined to be greater than 320.0 mg/l

Executive summary:

The toxicity of FAT 36034/D to activated sludge was determined in a 3-hours respiration inhibition test according to the OECD Guideline No. 209, and to the EEC Method No. L 133/118. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations. The test substance concentrations tested were 320, 100, 32 and 10 mg/l. The reference substance, 3,5-Dichlorophenol was tested in concentrations of 32, 10 and 3.2 mg/l. IC50 (3 h) of FAT 36034/D is determined to be greater than 320.0 mg/l while the IC50 (3 h) of the reference substance 3,5-Dichlorophenol is 13.0 mg/l

Description of key information

IC50 (3 h) of FAT 36034/D is determined to be greater than 320.0 mg/l 

Additional information

A study was performed to determine the toxicity of FAT 36034/D to activated sludge in a 3-hours respiration inhibition test according to the OECD Guideline No. 209, and to the EEC Method No. L 133/118. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations. The test substance concentrations tested were 320, 100, 32 and 10 mg/l. The reference substance, 3,5-Dichlorophenol was tested in concentrations of 32, 10 and 3.2 mg/l. IC50 (3 h) of FAT 36034/D is determined to be greater than 320.0 mg/l.