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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Study period:
14 - 15 Oct 1993
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by solving 12513 mg a.i. in 1 L double distilled water. The test concentrations were prepared by a dilution series.
Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Initial biomass concentration: extinction of 0.140 at a wave length of 436 nm
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Remarks on exposure duration:
16 +/- 1 hour
Test temperature:
21.8 °C
pH:
before incubation: 5.6 - 6.2
after incubation: 6.8
Nominal and measured concentrations:
Nominal content of active substance: 313, 626, 1251, 2502.5, 5005 and 10010 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): closed
- Material, fill volume: glass, 100 mL test medium
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 5
- No. of vessels per abiotic control (replicates): 6

Reference substance (positive control):
no
Duration:
16 h
Dose descriptor:
EC50
Effect conc.:
> 10 g/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth inhibition
Duration:
16 h
Dose descriptor:
EC10
Effect conc.:
> 10 g/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth inhibition

Test Concentration [mg a.s./L]

Inhibition [%]

313

-6.9

626

-7.3

1251

-4.7

2502.5

-2.9

5005

-0.9

10010

7.4

Description of key information

The effect of Alcohols, C12-14, ethoxylated, sulfates, sodium salts on the growth of Pseudomonas putida was investgated in a GLP guideline studyaccording to DIN 38412, part 8. An EC10 and an EC50 > 10 g/L were determined after approximately 16 h exposure.

Key value for chemical safety assessment

Additional information

The toxicity of the alkyl ether sulfates (AES) substances to microorganisms was assessed by read-across based on the grouping of substances (category approach). All data on toxicity to microorganisms available for the category were used to assess the toxicity of category substances to microorganisms. The available data indicate no adverse effects of alkyl ether sulfates on microbial activity. All substances of the alkyl ether sulfates category are considered readily biodegradable. In the available screening studies including a toxicity control, no inhibition of the degradation process by the test substance was observed. The available studies investigating the respiration inhibition of activated sludge organisms and Pseudomonas putida determined no detrimental effects of the tested alkyl ether sulfates.