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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin irritation / corrosion

Currently viewing:

Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Not applicable
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable study report which meets basic scientific principles
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1957

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
BASF test according to internal SOP
GLP compliance:
no
Remarks:
Conducted prior to GLP guidelines

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2'-iminodi(ethylamine)
EC Number:
203-865-4
EC Name:
2,2'-iminodi(ethylamine)
Cas Number:
111-40-0
Molecular formula:
C4H13N3
IUPAC Name:
bis(2-aminoethyl)amine
Details on test material:
- Name of test material (as cited in study report): Diaethylentriamin (as named in study report); Substance ID: VI/236

Test animals

Species:
rabbit
Strain:
other: white rabbit
Details on test animals or test system and environmental conditions:
No data

Test system

Type of coverage:
not specified
Preparation of test site:
not specified
Vehicle:
water
Controls:
not specified
Amount / concentration applied:
- Concentration (if solution): 40% and 100%
Duration of treatment / exposure:
1, 5, and 15 min
Observation period:
3 weeks
Number of animals:
2 animals used
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes, lutrol concentrated and 50% dilution in water used
- Time after start of exposure: 1, 5, 15 mins

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
other: necrosis
Basis:
animal #1
Time point:
other: 15 min
Remarks on result:
other: 100% Diethylenetriamine; upon substance removal very strong reddening with begining necrosis
Irritation parameter:
erythema score
Basis:
animal #1
Time point:
other: 15 min
Score:
0
Max. score:
0
Remarks on result:
other: 40% of diethylene triamine; No findings
Irritation parameter:
erythema score
Basis:
animal #2
Time point:
other: 15 min
Reversibility:
no data
Remarks on result:
other: 40% of diethylene triamine; visible erythema
Irritation parameter:
erythema score
Basis:
mean
Time point:
other: 1 and 5 min
Score:
0
Remarks on result:
other: 40% of diethylene triamine ; without findings
Irritation parameter:
other: necrosis
Basis:
animal #1
Time point:
other: 15 min
Remarks on result:
other: 100% of diethylene triamine; upon substance removal very strong reddening with begining necrosis
Irritation parameter:
other: necrosis
Basis:
animal #1
Time point:
other: Day 1
Remarks on result:
other: 100% of diethylene triamine; On day 1 greyish-brownish necrosis
Irritation parameter:
other: necrosis
Basis:
animal #1
Time point:
other: Day 2
Remarks on result:
other: 100% diethylenetriamine; On day 2 leather-like blackish-brown crust-formation
Irritation parameter:
other: necrosis
Basis:
animal #1
Time point:
other: 3 weeks
Reversibility:
not reversible
Remarks on result:
other: 100% Diethylenetriamine; deep scar 3 weeks later
Irritation parameter:
other: necrosis
Basis:
animal #2
Time point:
other: 15 min
Remarks on result:
other: 100% Diethylenetriamine; blackish-brown necrosis, bleeding, marginal red swelling
Irritation parameter:
other: necrosis
Basis:
animal #2
Time point:
other: 15 min
Reversibility:
no data
Remarks on result:
other: 100% of diethylene triamine; blackish-brown necrosis, bleeding, marginal red swelling
Irritant / corrosive response data:
Refer to remarks below
Other effects:
Refer to remarks below

Any other information on results incl. tables

40% of diethylene triamine

15 min exposure:

1 animal without findings, one animal visible erythema

5 min exposure: without findings

1 min exposure: without findings

100% of diethylene triamine:

15 minutes exposure:

1st animal: upon substance removal very strong reddening with begining necrosis. On day 1 greyish-brownish necrosis; on day 2 leather-like blackish-brown crust-formation with remaining deep scar 3 weeks later

2nd animal: blackish-brown necrosis, bleeding, marginal red swelling

5 min of exposure:

1st animal: upon substance removal: reddening, spotted necrosis; on day 1 grey-brownish necrosis; on day 2 leather-like blackish-brown crust formation with remaining deep deep scar 3 weeks latter.

2nd animal ; upon substance removal; Tan necrosis, bleeding, marginal red swellling; on day 1 anaemic necrosis with marginal reddening; 8 day hard crust formation with remainning scar after 3 weeks

1 min of exposure: upon substance removal slight reddening, partly necrotic wtih marginal reddening; on day 1 slight reddening and pea sized necrosis; On day 2 spotted reddening with pea-sized necrosis; on day 8 spotted crusts with remaining scars.

Applicant's summary and conclusion

Conclusions:
A 15 minute exposure to DETA resulted in necrosis in two of two rabbits.
Executive summary:

The skin irritation potential of DETA was examined. A 15 minute exposure to DETA resulted in necrosis in two of two rabbits.