Registration Dossier
Registration Dossier
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EC number: 231-943-8 | CAS number: 7779-88-6
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Remarks:
- Hen´s Egg Test on the Chorioallantoic Membrane (HET-CAM)
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Remarks:
- According to GLP and to Alternative methods currently available to the Draize rabbit eye test
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Invittox 1992 protocol no 47: HET-CAM test
- Principles of method if other than guideline:
- Hen´s Egg Test Chorioallantoic Membrane (HET-CAM) test permits the identification of irritative reactions which appear to be similar to those which occur in the eye using the standard Draize rabbit eye test. In the HET-CAM test system, three reactions are determined: haemorrhage, lysis and coagulation of the chorio-allantoic membrane at the ninth day of embryonation when nerve tissue and pain perception have not yet developed.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Zinc nitrate
- EC Number:
- 231-943-8
- EC Name:
- Zinc nitrate
- Cas Number:
- 7779-88-6
- Molecular formula:
- HNO3.1/2Zn
- IUPAC Name:
- zinc nitrate
- Test material form:
- solid: crystalline
Constituent 1
Test system
- Vehicle:
- water
- Controls:
- yes, concurrent positive control
- Amount / concentration applied:
- 0.3ml of the 10% mixture of the test article (1g of test article was mixed with distilled water to a total volume of 10ml)
- Duration of treatment / exposure:
- 5 minutes
- Duration of post- treatment incubation (in vitro):
- 5 minutes
- Number of animals or in vitro replicates:
- 24 White Leghorn eggs
Results and discussion
In vitro
Resultsopen allclose all
- Irritation parameter:
- in vitro irritation score
- Run / experiment:
- 10% zinc nitrate hexahydrate
- Value:
- 0
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- in vitro irritation score
- Run / experiment:
- positive control: 0.1N NaOH
- Value:
- 13.23
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- positive indication of irritation
- Irritation parameter:
- in vitro irritation score
- Run / experiment:
- positive control: 1% SDS
- Value:
- 8.6
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- positive indication of irritation
- Irritation parameter:
- in vitro irritation score
- Run / experiment:
- vehicle: distilled water
- Value:
- 0
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- no indication of irritation
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Remarks:
- test is one of the non-OECD tests not recommended for use under REACH
- Conclusions:
- Based on the threshold concentration of greater than 10% and the IS10% of 0, the irritating potential of zinc nitrate hexahydrate was determined as none to slight.
- Executive summary:
The aim of this study is to evaluate the potential ocular irritancy of a test substance as measured by its ability to induce toxicity in the chorioallantoic membrane of a hen. Effects are measured by the onset of: (1) hemorrhage; (2) coagulation; and (3) vessel lysis. These assessments are considered individually and then combined to derive a score, which is used to classify the irritancy level of the test substance.
Based on the threshold concentration of greater than 10% and the IS10% of 0, the irritating potential of zinc nitrate hexahydrate was determined as none to slight.
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