Registration Dossier
Registration Dossier
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EC number: 204-661-8 | CAS number: 123-91-1
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The substance is with high probability acutely not harmful to fish.
Key value for chemical safety assessment
Marine water fish
Marine water fish
- Effect concentration:
- 6 700 mg/L
Additional information
For the assessment of the short-term toxicity of 1,4-dioxane to fish experimental data are available. As a key study a prolonged GLP study using Oryzias latipes as test species was used. This flow-through study revealed a 21-d LC50 being above the highest test concentration (100 mg/L). The test concentrations were analytically verified and deviated less than 20% from the nominal values (data published, J-CHCK database, Japan, 2020).
This result is supported by a number of other studies. These studies are of varying quality, mostly not being carried out strictly to current guidelines and GLP. Several fish species including also marine species have been tested. In a non GLP and non-guideline marine study with individuals of inland silversides (Menidia beryllina) a 96-h LC50 of 6700 mg/L was reported (Dawson et al. 1975/77).
Moreover, two acute toxicity tests with Pimephales promelas as a test organism are available for the substance. These tests were performed according to special acute fish toxicity test system by the Center for Lake Superior Environmental Studies, University of Wisconsin-Superior (Geiger et. all, 1990). The 96-h LC50 values were determined to be 10800 and 9850 mg/L (nominal, analytically verified).
In addition, a static test conducted according to an internal test method “Fish Acute Toxicity test” with Pimephales promelas is also available for the substance. In this study the 96-h LC50 was determined to be 13000 mg/L (nominal, analytically verified, Dow Chemical, TSCAT, 1989).
Overall it can be concluded that the 96-h LC50 is higher than 100 mg/L as a worst-case consideration. Therefore, based on the available experimental data it can be concluded that the substance is with high probability acutely not harmful to fish.
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