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EC number: 208-762-8
CAS number: 540-97-6
Number of immobilised daphnids as compared to the number
exposed: Number of immobilised daphnids: 2, Number
exposed: 70 (includes control and solvent control).
Concentration response with 95% confidence limits: > 4.6
µg/L (empirically estimated) for survival and reproduction,
corresponding 95% confidence intervals could not be calculated.
- Survival: 90 and 100% survival
was observed in the
control and solvent control, respectively (pooled control =
95%). Survival of 100, 90, 100, 100 and 100% was observed
among daphnids exposed to the 0.27, 0.57, 1.2, 2.5 and 4.6
µg/L treatment levels, respectively.
Organisms exposed to the control and solvent control
released a mean cumulative offspring per female of 165 and
148, respectively (pooled control = 156 offspring per
female). Organisms exposed to the 0.27, 0.57, 1.2, 2.5 and
4.6 µg/L treatment levels released a mean cumulative
offspring per female of 158, 151, 141, 149 and 155, respectively.
Total body length at test termination among control and
solvent control daphnids averaged 4.91 and 4.84 mm per
daphnid, respectively (pooled control = 4.88 mm). Total
body length among daphnids exposed to the 0.27, 0.57, 1.2,
2.5 and 4.6 µg/L treatment levels averaged 4.86, 4.85, 4.84,
4.84 and 4.91 mm, respectively.
Dry weight at test termination among control and solvent
control daphnids averaged 1.06 and 1.12 mg per daphnid,
respectively (pooled control = 1.09 mg). Dry weight among
daphnids exposed to the 0.27, 0.57, 1.2, 2.5 and 4.6 µg/L
treatment levels averaged 1.10, 1.15, 1.11, 1.17 and 1.18 mg
per daphnid, respectively.
Daphnia reproduction: (21 day): NOEC: ≥4.6 µg/l, effects of the
test substance on survival, growth and reproduction of Daphnia magna.
Reliable test results are available for long-term toxicity to aquatic
A 21-day NOEC of ≥4.6 µg/l has been determined for the effects of the
test substance on survival, growth and reproduction of Daphnia magna in
accordance with OECD TG 211 and in compliance with GLP (Springborn
Smithers, 2009). The results have been obtained under semi-static
conditions and are expressed relative to geometric mean measured
concentrations of the substance.
In view of the hydrolysis half-life of the test substance and the semi
static exposure regime, it is likely that the test organisms were
exposed primarily to the tested substance rather than its degradation
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