Triethoxy(vinyl)silane

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993-06-01 to 1993-06-03

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

The concentration of hydrolysis products in stock solution determined by Total Organic Carbon analysis. The stock solution concentration (464 mg/L equivalent to a test substance concentration of 1145 mg/L) was used as the basis for determining the concentrations in the test media.

Vehicle:

no

Details on test solutions:

- Method: Test media prepared by dilution of a 1000 mg/L stock solution prepared by mixing for 18 h followed by filtration. TOC concentration of stock solution determined.

- Treatments: Control and nominal test concentrations of 57, 80, 115, 160, 229, 321 and 458 mg/L.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM

- Common name: Daphnia magna

- Strain: Clone 5

- Source: Stock culture

- Age at study initiation (mean and range, SD): <24 h

- Method of breeding: Parthenogenetic reproduction

- Feeding during test: None


ACCLIMATION

- Acclimation conditions (same as test or not): yes

- Type and amount of food: sufficient to be consumed each day leaving no residue

- Feeding frequency: daily

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Hardness:

The sum of the Ca2+ and Mg2+ ions was 2.5 mmol

Test temperature:

20 ±1ºC

pH:

7.0 - 7.2

Dissolved oxygen:

6.5 - 8.1 mg/L

Salinity:

Not applicable

Nominal and measured concentrations:

Control and nominal test concentrations of 57, 80, 115, 160, 229, 321 and 458 mg/L.

Details on test conditions:

TEST SYSTEM

- Test vessel:

- Type: open

- Material, size, headspace, fill volume: glass cylinder containing 10 ml of test medium

- Aeration: none

- No. of organisms per vessel: 5

- No. of vessels per concentration (replicates): 4

- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: Elendt M4 medium

- Intervals of water quality measurement: end of test


OTHER TEST CONDITIONS

- Photoperiod: Dark


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilization after 24 and 48 h exposure

TEST CONCENTRATIONS

- Spacing factor for test concentrations: 1.4


STATISTICS

EC50 and confidence interval determined by Probit analysis

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

297.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 223.9 - 224.2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

168.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 126.9 - 224.2

Details on results:

- Mortality of control: 0

Results with reference substance (positive control):

- EC50: >0.9, <1.9 mg/L

Reported statistics and error estimates:

The EC50 value and its 95% confidence interval was calculated by Probit analysis.

Table 1. Test results

Nominal test substance concentration (mg/L)	Percentage immobile Daphnia after 24 hours	Percentage immobile Daphnia after 48 hours
0 (Control)	0	0
57	5	25
80	5	25
115	5	35
160	0	35
229	25	45
321	55	75
458	90	100

Validity criteria fulfilled:

yes

Conclusions:

Measured data was not available for trieoxy(vinyl)silane (CAS 78-08-0) data was therefore read across from the comparable substance, Trimethoxyvinylesilane (CAS 2768-02-7). A 48-h EC50 value of 168.7 mg/L has been determined for the effects of the test substance on mobility of Daphnia magna. A NOEC was not determined in the test. It is likely that the test organisms were primarily exposed to the hydrolysis products of the substance.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

Not reported

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions. No GLP or analysis of exposure media and no description of the test substance.

Principles of method if other than guideline:

Not described

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

no data

Test organisms (species):

Daphnia magna

Details on test organisms:

Not reported

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

Hard water

Test temperature:

23±1ºC

pH:

no data

Dissolved oxygen:

no data

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal concentrations: 0(Control), 1, 10 and 100 mg/l

Details on test conditions:

TEST SYSTEM

- Test vessel: Beaker

- Type: open

- Material, size, fill volume: Glass, 200 ml with 200 ml of test medium

- Aeration: not reported

- Renewal rate of test solution: not reported

- No. of organisms per vessel: 10

- No. of vessels per concentration (replicates): 2

- No. of vessels per control (replicates): 2

- Biomass loading rate: 1 daphnid/20 ml


TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: Reconstituted hard water prepared from distilled water.

- Culture medium different from test medium: no

- Intervals of water quality measurement: not reported


OTHER TEST CONDITIONS

- Adjustment of pH: no data

- Photoperiod: 18 h light, 6 h dark

- Light intensity: 600 foot candles


EFFECT PARAMETERS MEASURED: daily mortalities

TEST CONCENTRATIONS

- Spacing factor for test concentrations: 10

- Range finding study: not reported

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality of control: 5%

- Other adverse effects control: none

Reported statistics and error estimates:

There were insufficient mortalities in the test to determine an LC50 value. Statistical analysis was not applied to determining a NOEC..

20% mortality was recorded in the 100 mg/l treatment.

Validity criteria fulfilled:

yes

Conclusions:

A 48-hour EC50 value of >100 mg/l and NOEC of 1 mg/l have been determined for the effects of the test substance on mobility of Daphnia magna. It is likely that the test organism were primarily exposed to the hydrolysis product of the substance.

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

Not reported

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Secondary literature

Principles of method if other than guideline:

Not described

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Test type:

not specified

Water media type:

freshwater

Dose descriptor:

EC50

Effect conc.:

360 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

mobility

Validity criteria fulfilled:

not specified

Conclusions:

An EC50 value of 360 mg/l has been determined for the effects of the test substance on mobility of Daphnia magna. The result is reported in secondary literature and its reliability cannot be assessed.

Reference 4

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to overall summary (IUCLID Section 6.1) and attached justification for grouping in IUCLID Section 13.

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

168.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 126.9 - 224.2

Remarks:

Hüls AG 1993

Dose descriptor:

EC50

Effect conc.:

360 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

mobility

Remarks on result:

other: Chandra 1997

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Dow Corning 1979

Description of key information

A 48-h EC50 value of 168.7 mg/l (mobility of Daphnia magna, EU Method C.2), RL2

Additional information

Measured data were not available for the target substance triethoxy(vinyl)silane (CAS 78-08-0), therefore read across from the analogue substance, trimethoxy(vinyl)silane (CAS 2768-02-7) was deemed acceptable for assessment.

According to EU Method C.2 and GLP a 48 h EC50 value of 168.7 mg/l was determined for the effects of the test substance on mobility of Daphnia magna (Hüls AG, 1993a). Further information support that the substance is not toxic to freshwater invertebrates (Chandra, 1997). As the test substance hydrolyses rapidly in water it is likely that the test organisms were exposed predominantly to its hydrolysis products.