Acetic acid

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: The study report has not been received and therefore cannot be reviewed and evaluated in full.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD 202-II

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Acetic acid

EC Number:

200-580-7

EC Name:

Acetic acid

Cas Number:

64-19-7

Molecular formula:

C2H4O2

IUPAC Name:

acetic acid

Details on test material:

Acetic acid technical 60%. Lot/batch A07ET265, Purity not stated.

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No further details

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Counting of new-born living daphnia and living adults. Average oxygen content, aaerage pH, substnace concentration and temperature were checked at the beginning 3 time weekly after test initiation and 21 days after test initiation. Effects of test substnace observed daily.

Test solutions

Vehicle:

no

Details on test solutions:

Test solutionsrenewed 8 times in the exposure period.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

2-24h old

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Post exposure observation period:

Not applicable

Test conditions

Hardness:

Not reported

Test temperature:

20°C±1

pH:

7.52-7.98

Dissolved oxygen:

7.8-8.6mg/l

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal concentrations: 0, 84, 88, 93, 97, 102, 107 mg/l. Measured concentrations (time-weighted mean): 0, 52.4, 47.9, 54.3, 65.5, 73.8, 59.7mg/l.

Details on test conditions:

Daphnia fed green algae ( Scenedesmus subspicatus).
Concentrations tested with 8 replicates.
40 animals divided into groups of 10 animals in each test concentration and control

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

80 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: 60% acetic acid

Effect concentrations 2

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

31.4 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: For 100% acetic acid

Details on results:

Recovery rates of acetic acid in the test medium were below 80% of nominal before water renewal. EC50 values calculated with nominal and with measured (TWM) concentrations.

Results with reference substance (positive control):

not reported

Reported statistics and error estimates:

Determination of EC50 was carried out by probit analysis.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The NOEC for reproduction, based on mean measured concentrations, was reported to be 80mg/l for 60% acetic acid. The NOEC for reproduction, based on mean measured concentrations, was determined to be to be 31.4mg/l for 100% acetic acid.

Executive summary:

The NOEC for reproduction, based on mean measured concentrations, was determined to be to be 31.4mg/l for 100% acetic acid. This study is GLP compliant and follows a standard guideline. It is considered suitable for use as a key study.