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EC number: 932-349-8 | CAS number: 8006-64-2
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Justification for type of information:
- Crude sulfate turpentine is a UVCB substance that contains the same main constituents as gum turpentine oil. Therefore, data on crude sulfate turpentine can be extrapolated to gum turpentine oil. See read-across justification document in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- 29 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- water-accommodated fraction loading rate
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- water-accommodated fraction loading rate
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: none
- Mortality of control: 0
- Other adverse effects control: none - Reported statistics and error estimates:
- Determination of the LL50 (LL = Lethal Loading) of the lethality in the Fish Test was accomplished using Probit-analysis (Finney-method, lognormal distribution; confidence limit 95 %, significance level: 0.05). The NOELR was determined directly from the raw data.
- Sublethal observations / clinical signs:
Table 1. Test results - percentage mortality for each observation period
Time [h]
Control
2.5 mg/L
5.0 mg/L
10 mg/L
25 mg/L
50 mg/L
Number
[%]
Number
[%]
Number
[%]
Number
[%]
Number
[%]
Number
[%]
24
0/7
0
0/7
0
0/7
0
0/7
0
0/7
0
1/7
14.3
48
0/7
0
0/7
0
0/7
0
0/7
0
0/7
0
7/7
100
72
0/7
0
0/7
0
0/7
0
0/7
0
0/7
0
7/7
100
96
0/7
0
0/7
0
0/7
0
0/7
0
1/7
14.3
7/7
100
Table 2. Results of TOC analysis of test media (mg/L)
Period
Control
2.5 mg/L
10 mg/L
50 mg/L
0-24 h start
1.3
1.0
*
2.9
0-24 h end
0.8
0.7
1.2
3.0
72-96 h start
1.5
1.6
1.9
-
72-96 h end
1.4
1.3
1.8
-
Table 4. Results of GC-MS analysis of test media
Vessel
Time [h]
α-Terpineol
[µg/L]α-Pinene
[µg/L]β-Pinene
[µg/L]3-Carene
[µg/L]Limonene
[µg/L]Total
[µg/L]
Control
0 - 24 start
0.214**
< 0.367*
0.227**
< 0.151*
0.212**
< 1.17
Control
0 - 24
end
0.214**
< 0.367*
0.227**
< 0.151*
0.212**
< 1.17
Control
72 -96 start
0.214**
< 0.367*
0.227**
< 0.151*
0.212**
< 1.17
Control
72 - 96 end
0.214**
< 0.367*
0.227**
< 0.151*
0.212**
< 1.17
2.5 mg/L
0 - 24 start
2.27
< 0.367*
0.227**
0.625
0.463
< 3.95
2.5 mg/L
0 - 24
end
1.74
0.636
0.227**
0.437
0.212**
3.25
2.5 mg/L
72 -96 start
1.51
1.03
0.227**
0.596
0.414
3.78
2.5 mg/L
72 - 96 end
1.47
0.754
0.227**
0.564
0.372
3.39
10 mg/L
0 - 24 start
14.0
34.0
2.78
9.49
4.11
64.4
10 mg/L
0 - 24
end
7.72
23.4
1.86
5.57
2.36
40.9
10 mg/L
72 -96 start
6.11
34.5
3.82
11.7
5.07
61.2
10 mg/L
72 - 96 end
6.18
36.5
3.32
12.1
5.01
63.1
50 mg/L
0 - 24 start
43.1
405
31.6
122
47.9
649
50 mg/L
0 - 24
end
42.6
396
20.2
80.3
33.6
573
Remarks:
* Limit of detection
** derived from equation of the calibration line (axis intercept); no signal was measured in the controls for α-Terpineol, β-Pinene and Limonene
Quantitative GC-MS-values are based on results and/or limits of detection
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 96-hour LL50 value of 29 mg/L and a NOELr of 5 mg/L have been determined for the effects of the tested substance on mortality of Danio rerio. The fish were exposed to water-accommodated fractions of the substance.
- Executive summary:
An acute aquatic toxicity study was performed on zebrafish (Danio rerio) in accordance with GLP and OECD Guideline 203. Groups of seven fish were exposed to Water Accommodated Fractions (WAFs) of the test item over a range of nominal loading values of 2.5, 5, 10, 25 and 50 mg/L. The total test period was 96 h. The mortality of the fish was determined in a semi-static 96-h test by visual observation after 24, 48, 72 and 96 h.
After the 96 h of exposure, mortality was 0% at 2.5, 5 and 10 mg/L (loading), 14.3% at 25 mg/L (loading) and 100% at 50 mg/L (loading).
Therefore, the 96h LL50 for the test item to zebrafish (Danio rerio) was estimated to be 29 mg/L and. The 96h NOELr for the test item to zebrafish (Danio rerio) was estimated to be 5 mg/L.
Reference
Description of key information
A 96-h LL50 of 29.0 mg/L and NOELR of 5.0 mg/L have been determined for the effect of Crude sulfate turpentine on mortality of Danio rerio.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 29 mg/L
Additional information
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