2-(2-(2-methoxyethoxy)ethoxy)ethanol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19 Feb 1974 - 01 Mar 1974

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable study report which meets basic scientific principles

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

no

Principles of method if other than guideline:

Method: BASF-Test which follows in principle the methods described in OECD Guideline 401.
5 rats per sex and dose were treated simultaneously by gavage with preparations of the test substance in water. Group-wise documentation of clinical signs was performed over the 7 day study period. The clinical signs and findings were reported in summary form.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-(2-(2-methoxyethoxy)ethoxy)ethanol

EC Number:

203-962-1

EC Name:

2-(2-(2-methoxyethoxy)ethoxy)ethanol

Cas Number:

112-35-6

Molecular formula:

C7H16O4

IUPAC Name:

2-[2-(2-methoxyethoxy)ethoxy]ethan-1-ol

Details on test material:

- Name of test material (as cited in study report): Methyltriglykol. Test substance No. 88/289
- Physical state: liquid
- Analytical purity: ca. 95%

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: male: 186g (mean), female 165g (mean)

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 35% for 10500 mg/kg and 15% for 1050 and 2257 mg/kg

Doses:

1050, 2257, 10500 mg/kg bw

No. of animals per sex per dose:

5 male and 5 female rats per dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: yes

Statistics:

On the basis of the observed lethality, the LD50 value was estimated or determined using a graphical evaluation of the dose response curve on probability paper.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 10 500 mg/kg bw

Mortality:

no mortality occured.

Clinical signs:

other: Signs of toxicity comprised at 10500 mg/kg: apathy, spastic gait, dyspnea, mouth and eyelid crust formation.

Gross pathology:

At necroscopy no abnormalities were observed.

Any other information on results incl. tables

Mean Body weights (g) MALES:

Dose group (mg/kg)	Day 0	Day 3	Day 7
1050	190	210	205
2257	199	228	222
10500	169	124	159

 

Mean Body weights (g) FEMALES:

Dose group (mg/kg)	Day 0	Day 3	Day 7
1050	165	184	167
2257	163	178	170
10500	168	192	178

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Executive summary:

In an acute oral toxicity study that followed the basic requirements of the OECD standard protocol guideline, male and female rats were exposed to single gavage doses of 2 -(2 -(2 -methoxyethoxy)ethoxy)ethanol at doses up to 10500mg/kg. The high dose animals showed apathy, spastic gait, dyspnea, mouth and eyelid crust formation and males also showed a sharp but transient drop in body. However, no mortality was reported implying that the LD50 is significantly in excess of the maximum dose tested.

Synopsis

LD50 (male, female) >10500mg/kg