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Toxicity to microorganisms

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Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
No GLP, no analytical monitoring. Concentrations of the test substance were several orders of magnitude above the limit of solubility. There is, however, no indication that the undissolved test substance interfered with bacterial respiration or the functioning of the test system.
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Principles of method if other than guideline:
Respiration Inhibition Test: according to Offhaus (5d).
GLP compliance:
no
Specific details on test material used for the study:
Supplier: Evonik Röhm GmbH, D-64293 Darmstadt, Germany
Analytical monitoring:
no
Test organisms (species):
activated sludge
Test type:
static
Limit test:
no
Total exposure duration:
5 d
pH:
adjusted to pH: 7.0
Nominal and measured concentrations:
100, 200 and 500 mg/L emulsion
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed system
Duration:
5 d
Dose descriptor:
EC0
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Conclusions:
No inhibition of respirometric activity in any of the tested concentrations. LC0 (bacteria): > 500 mg/L in emulsion according to OECD 209.
Executive summary:

No inhibition of respirometric activity in any of the tested concentrations. LC0 (bacteria): > 500 mg/L in emulsion according to OECD guideline 209.

NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
GLP Concentrations of the test substance were several orders of magnitude above the limit of solubility. There is, however, no indication that the undissolved test substance interfered with bacterial respiration or the functioning of the test system.
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Principles of method if other than guideline:
Five concentrations of the test material (1, 10, 100, 1000, 10000 mg/l) were tested. As the solubility of the substance was far exceeded and no
physical inhibitory effects (e.g. agglomeration of activated sludge) were observed, no further tests were performed.
GLP compliance:
yes
Specific details on test material used for the study:
- Name of test material (as cited in study report): Isotridecyl methacrylate
Analytical monitoring:
yes
Details on sampling:
- Sample storage conditions before analysis: 20 °C +/- 2 °C
Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
- Pretreatment:
A sample of mixed liquor from the sewage plant was obtained and aerated during transportation to the laboratory.
Two samples of 10 ml were weighed and dried at 105 °C. The amount of solids was calculated and the wet sludge suspended with isotonic solution to yield a concentration of 3 g suspended solids per litre. Subsequently, the activated sludge was amended with 50 ml synthetic sewage per litre and aerated at 20 °C + 2 °C overnight.
Before use the amount of solids was determined and adjusted to 3 g / l. Additionally, the pH-value was controlled. An adjustment was not necessary as the pH was in the accepted range of 6.0 – 8.0
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
180 min
Test temperature:
20 °C +/- 2 °C
pH:
pH 6 - 8
Nominal and measured concentrations:
1, 10, 100, 1000 and 10000 mg/l
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
Five concentrations of the test material (1, 10, 100, 1000, 10000 mg/l) were tested. As the solubility of the substance was far exceeded and no
physical inhibitory effects (e.g. agglomeration of activated sludge) were observed, no further tests were performed.
Results with reference substance (positive control):
- Relevant effect levels:
The EC50 (3 hours) of 3,5-dichlorophenol was in the accepted range of 5 to 30 mg/l.

In every test the two control respiration rates were within 15 % of each other
Validity criteria fulfilled:
yes
Conclusions:
Isotridecyl methacrylate is of low toxicity to microorganisms relevant for biodegradation.
The EC50 (3 h) for the inhibition of biodegradation was > 10000 mg/L.
Executive summary:

Isotridecyl methacrylate was tested for toxicity to bacteria relevant for the biodegradation of the substance. In concentrations up to 10000 mg/l, which are far exceeding the water solubility of the substance, no inhibition of the oxygen consumption of the bacteria was observed within 180 min.

Therefore, it can be concluded that isotridecyl methacrylate is non-toxic to bacteria.

NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
GLP, no analytical monitoring. Purity of test substance is not reported. Concentrations of the test substance were several orders of magnitude above the limit of solubility. There is, however, no indication that the undissolved test substance interfered with bacterial respiration or the functioning of the test system.
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes
Analytical monitoring:
no
Test organisms (species):
activated sludge
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
> 10 000 mg/L
Conclusions:
The test substance Methacrylic acid methyl ester 13,6 is of low toxicity to microorganisms relevant for biodegradation.
The EC10 (3 h) for the inhibition of biodegradation was > 10000 mg/L.
Executive summary:

The test substance Methacrylic acid methyl ester 13,6 was tested for it's toxicity to bacteria relevant for the biodegradation of the substance. The EC10 (3 h) for the inhibition of biodegradation was > 10000 mg/L.

Therefore, it can be concluded that the test substance Methacrylic acid methyl ester 13,6 is non-toxic to bacteria.

NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.

Description of key information

No toxicity to bacteria was observed, the EC0 / EC50 values were, depending on the test conditions, between > 500 and > 10000 mg/L. Thus, concentrations of the test substances were several orders of magnitude above the limit of solubility. In conclusion, no inhibition of biodegradability in STPs or in the environment is to expect.

Key value for chemical safety assessment

Additional information

Several tests with activated sludge are available, all of them valid without or with minor restrictions only. The methacrylic acid esters tested had an alcohol moiety chain length of C12 – C22. No toxicity to bacteria was observed, the EC0 / EC50 values were, depending on the test conditions, between > 500 and > 10000 mg/L. Thus, concentrations of the test substances were several orders of magnitude above the limit of solubility. There is, however, no indication that the undissolved test substances interfered with bacterial respiration or the functioning of the test system.

In conclusion, no inhibition of biodegradability in STPs or in the environment is to expect.