Polysulfides, bis[3-(triethoxysilyl)propyl]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-12-02 to 1991-12-13

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Guideline study with acceptable restrictions. The study was conducted according to an appropriate OECD test guideline, with acceptable restrictions. The restriction was that the presence of undissolved test material (turbidity) in the water-accommodated fractions may have contributed to effects observed in the test.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION

- Method: Test media were prepared at nominal loading rates of 1000 and 10000 mg/L by adding appropriate amounts of the test substance to 1.5 litres of the dilution water and stirring for approximately 20 hours at a temperature of 25ºC. The resulting media were allowed to stand for 4 hours before introducing the fish. Fresh test media were prepared for daily renewal.

- Controls: Dilution water

- Evidence of undissolved material (e.g. precipitate, surface film, etc): It is likely that the solubility of the substance in the test medium was exceeded a
t the tested loading rates. Undissolved test substance was observed to be present in the test media.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM

- Common name: Zebrafish

- Source: Commercial hatchery; M.B. Ruysbroek B.V., Maassluis, Netherlands

- Length at study initiation (length definition, mean, range and SD): 2.1 +/-0.1 cm

- Weight at study initiation (mean and range, SD): 0.07 +/-0.01 g

- Feeding during test: none

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

204 mg/L

Test temperature:

24.3-25.6ºC

pH:

7.7-8.2

Dissolved oxygen:

≥5.6 mg/L

Nominal and measured concentrations:

Nominal loading rates: 0(Control), 1000 and 10000 mg/L.

It is likely that the solubility of the substance in the test medium was exceeded at the tested loading rates. Undissolved test substance was observed to be present in the test media.

Details on test conditions:

TEST SYSTEM

- Test vessel: Beaker

- Type (delete if not applicable): open

- Material, size, headspace, fill volume: glass, 2 litre with 1.5 litres of test medium

- Aeration: yes

- Renewal rate of test solution (frequency/flow rate): daily

- No. of organisms per vessel: 10

- No. of vessels per concentration (replicates): 2

- No. of vessels per control (replicates): 2

- Biomass loading rate: 0.5 g/L

TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: DSWL standard water prepared by adding salts to groundwater obtained from near Linschoten, Netherlands

- Total organic carbon: 1.7 mg/L

- Ca/Mg ratio: 1.32 mmol/L Ca2+ : 0.72 mmol/L Mg2+

- Culture medium different from test medium: no data

- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS

- Adjustment of pH: no

- Photoperiod: 16 h light 8 h dark with 30 minute transition

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality after 24, 48, 72 and 96 hours

TEST CONCENTRATIONS

- Spacing factor for test concentrations: 10

- Justification for using less concentrations than requested by guideline: not reported

- Range finding study: yes but results not reported

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOELR

Effect conc.:

>= 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: none

- Mortality of control: 0

- Other adverse effects control: none

- Effect concentrations exceeding solubility of substance in test medium: The solubility of the substance in the test medium (approximately 0.5 mg/l)was exceeded at the tested loading rates. Undissolved test substance was observed to be present in the test media.

Reported statistics and error estimates:

There were no effects in any treatment. Statistical analysis of the results was therefore not required.

Sublethal observations / clinical signs:

There were no toxic effects in either treatment.

Validity criteria fulfilled:

yes

Conclusions:

A 96-hour LL50 value of >10000 mg/L and NOELR of ≥10000 mg/L have been determined for the effects of the test substance on mortality of Brachydanio rerio. The water solubility of the substance (<1 mg/L) was exceeded at the tested loading rates and undissolved test substance was observed to be present in the test media.

Description of key information

LL₅₀ (96 h): >10000 mg/l test material (nominal, highest concentration tested), based on the mortality of Danio rerio (OECD Guideline 203 (Fish, Acute Toxicity Test)).

Additional information

A 96-hour LL₅₀ value >10000 mg/l (nominal) (highest concentration tested) have been determined for the effects of bis[3-(triethoxysilyl)propyl]polysulfides (CAS 211519-85-6, EC 915-673-4) on the mortality of Danio rerio in a test conducted according to OECD TG 203 and in compliance with GLP (Evonik Degussa, 1992b). The water solubility of the substance (<1 mg/l, measured; 0.01 - 0.014 mg/l, estimated) was exceeded at the tested loading rates and undissolved test substance was observed to be present in the test media. It is likely that, because of the prolonged test medium preparation phase, the test organisms were exposed to a mixture of the parent constituents and the hydrolysis products of the constituents, as well as undissolved test substance and/or precipitated oligomeric by-products.