Addition of EDTA alone to the growth medium increased EDTA chelation of most divalent cations, resulting in decreased plant uptake of Fe, Mn and Zn. EDTA application however, did not induce any antioxidative stress response. Addition of 200 µM EDTA resulted in a free EDTA concentration of 10 µM in solution.Obviously this was too low to induce any phytotoxic effects.
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EC number: 205-358-3 | CAS number: 139-33-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- seedlings of Phaseolus vulgaris were exposed to EDTA for 14 days
- GLP compliance:
- no
Test material
- Reference substance name:
- Edetic acid
- EC Number:
- 200-449-4
- EC Name:
- Edetic acid
- Cas Number:
- 60-00-4
- Molecular formula:
- C10 H16 N2 O8
- IUPAC Name:
- 2,2',2'',2'''-(ethane-1,2-diyldinitrilo)tetraacetic acid
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test substrate
- Vehicle:
- no
Test organisms
- Species:
- Phaseolus vulgaris
- Plant group:
- Dicotyledonae (dicots)
Study design
- Test type:
- seedling emergence toxicity test
- Study type:
- laboratory study
- Substrate type:
- other: seedlings grown in polystyrene squares
- Limit test:
- no
- Total exposure duration:
- 14 d
Test conditions
- Details on test conditions:
- Bean seeds received a cold treatment (+ 4 °C) for 3 days to break dormancy and to synchronize germination. They were transferred to a growth chamber to germinate between two layers of water-soaked rock wool for 4 days. Subsequently seed coats were removed and seedlings with a root length of approximately 1.5 cm were grown in 3-mm thick polystyrene squares by fixing the roots through 5-mm holes (9 plants/treatment). The polystyrene was floated on 3 L of aerated full strengths Hoagland's solution (pH 5.0) in 3.5 L polyethylene beakers; Fe was added as FeSO4 * 7H2O. To keep Fe in solution, it was complexed with EDTA (Fe/EDTA ratio 2/1). Nutrient solution was replaced every 2 days; pH was monitored daily. Plants were grown under controlled conditions in a 12-h photoperiod at 65 % relative humidity and day/night temperatures of 22°C. Light was supplied by cool white fluorescent lamps (L = 140W/20SA; Osram, Augsburg, Germany) at a photo synthetic photon flux density of 165 µmol/m2 * s at the leaf level. Final concentrations of 0 (= untreated), 50 and 200 µM EDTA in Hoagland's medium, equivalent to 14.6 and 58.4 mg/kg, respectively. Plants were harvested 14 days after the start of treatment, and morphological parameters (root weight, shoot length, leaf weight) were measured.
- Nominal and measured concentrations:
- Nominal: 14.6 and 58.4 mg/L
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Species:
- Phaseolus vulgaris
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 58.4 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
Any other information on results incl. tables
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- In a 14 day exposure test EDTA showed no inhibitory effect on seedling emergence and growth of Phaseolus vulgaris up to a concentration of 58.4 mg/kg soil.
- Executive summary:
In a 14 day eposure test EDTA showed no inhibitory effect on seedling emergence and growth of Phaseolus vulgaris up to a concentration of 58.4 mg/kg soil.
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