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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2004
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report date:
2005

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
and EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
5 ml from all test solutions (except controls and 1,5/1,78) before and after each medium renewal and end of the test.

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Rainbow trout.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
158 mg CaCO3 / l
Test temperature:
between 13 and 17 °C and do not vary more the than 1 °C
pH:
6.0 - 9.0
Dissolved oxygen:
above 60 % of the air saturation value at the selected temperature
Salinity:
no
Nominal and measured concentrations:

See the table below (any information on materials and methods icl. tables.

Concentration mg / l number of fish
Limit test
control 0 7
treated LS 7
(LS: Limit of solubility obtained from an initial loading rate of 100 mg/L.)

Range finding test
treated LS / 1000 5
treated LS / 100 5
treated LS / 10 5

Definitive test
Group 1 0 10
Group 2 LS / 31.6 10
Group 3 LS / 17.8 10
Group 4 LS / 10.0 10
Group 5 LS / 5.62 10
Group 6 LS / 3.16 10
Group 7 LS / 1.78 10
Group 8 LS 10






Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
2.84 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
dissolved
Basis for effect:
mortality (fish)
Remarks on result:
other: 95 % CL: 1.45-4.56 mg/L
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
2.7 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
dissolved
Basis for effect:
mortality (fish)
Remarks on result:
other: 95 % CL: 1.45-4.56 mg/L
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
2.7 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
dissolved
Basis for effect:
mortality (fish)
Remarks on result:
other: 95 % CL: 1.45-4.56 mg/L
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2.7 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
dissolved
Basis for effect:
mortality (fish)
Remarks on result:
other: 95 % CL: 1.45-4.56 mg/L
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
4.56 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
dissolved
Basis for effect:
mortality (fish)
Remarks on result:
other: 95 % CL: 1.45-4.56 mg/L
Details on results:
--
Results with reference substance (positive control):
--
Reported statistics and error estimates:
--

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Under experimental conditions, the 96-hour LC50 in a semi static system was 2.70 mg/L for rainbow trout.