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EC number: 700-990-0
CAS number: -
This eye irritation test, comparable to
OECD Guideline 405, 1981, consists of a single instillation of 0.1.ml of
the undiluted test material (Phosflex 72B) into one eye of each of 9
rabbits. The treated eye was washed with water 20-30 seconds after
exposure in 3 rabbits and the eyes were left unwashed in the remaining 6
rabbits. The untreated eye of each animal served as a negative control.
The cornea, the iris, and the bulbar and palpebral conjunctivae were
observed at 24, 48, and 72 hours, and at 4 and 7 days after treatment.
The guidelines described by Draize (1965) were used to assign numerical
scores to the observed effects and to develop a weighted total score for
each animal for each day of observations. Phosflex 72B produced no
irritation in the washed and unwashed eyes of albino rabbits. Based on
the results and according to the EU criteria outlined in 67/548/EEC
and 1272/2008/EC (CLP/EU-GHS),
Phosflex 72B does not have to be classified and has no obligatory
labelling requirement for eye irritation.
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